Purification of saponin compounds in Bupleurum falcatum by solvent partitioning and preparative LC

Saponin compounds (saikosaponin c, a, and d) in Bupleurum falcatum were partially purified by solvent partitioning of the herbal extract using diethyl ether, distilled water, n-butanol, and acetone. After separation of the saponins by preparative LC, the purity of each saikosaponin was more than 94%...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2001-07, Vol.65 (7), p.1648-1651
Main Authors: Kim, N. (Korea Food Research Inst., Kyonggi (Korea R.)), Park, I.S
Format: Article
Language:English
Subjects:
Biological and medical sciences
BUPLEURUM
Bupleurum - chemistry
Chromatography, Liquid
General pharmacology
Medical sciences
Molecular Structure
Oleanolic Acid - analogs & derivatives
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
preparative LC
PURIFICATION
saikosaponins
Sapogenins - chemistry
Sapogenins - isolation & purification
SAPONINS
Saponins - isolation & purification
solvent partitioning
SOLVENTS
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Summary:Saponin compounds (saikosaponin c, a, and d) in Bupleurum falcatum were partially purified by solvent partitioning of the herbal extract using diethyl ether, distilled water, n-butanol, and acetone. After separation of the saponins by preparative LC, the purity of each saikosaponin was more than 94%. The identities of purified individual saikosaponins were confirmed by TLC, analytical LC, and fast-atom bombardment mass spectrometry. ---[End of Page 10]---
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.65.1648