Mast cell distribution and density in the normal uterus — metachromatic staining using lectins

This study investigates the number and the distribution of mast cells in the normal human uterus. Reliability of results was ensured by prompt tissue fixation and the use of biotin-labelled lectins in conjunction with the avidin–biotin peroxidase complex (ABC) method. This design revealed that mast...

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Bibliographic Details
Published in:European journal of obstetrics & gynecology and reproductive biology 2001-09, Vol.98 (1), p.109-113
Main Authors: Sivridis, E, Giatromanolaki, A, Agnantis, Niki, Anastasiadis, P
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Cell Count
Con A
Concanavalin A
Endometrium - cytology
Female
Fundamental and applied biological sciences. Psychology
Humans
Lectins
Mammalian female genital system
Mast cells
Mast Cells - cytology
Middle Aged
Morphology. Physiology
Myometrium
Myometrium - cytology
Normal endometrium
Phytohemagglutinins
Staining and Labeling
Uterus - cytology
Vertebrates: reproduction
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Summary:This study investigates the number and the distribution of mast cells in the normal human uterus. Reliability of results was ensured by prompt tissue fixation and the use of biotin-labelled lectins in conjunction with the avidin–biotin peroxidase complex (ABC) method. This design revealed that mast cells are, indeed, normal constituents of the human uterus. They occur in large numbers in the myometrium, but are only scanty in the endometrium where they tend to be confined to the stratum basalis. The mean mast cell counts per high power field (MC/HPF), after staining with Canavalia ensiformis agglutinin (Con A), were 17.9 MC/HPF in the inner half of the myometrium, and 8.3 MC/HPF in the outer half of the myometrium; 2.7 MC/HPF in the basalis, and 0.3 MC/HPF in the functionalis ( P
ISSN:0301-2115
1872-7654
DOI:10.1016/S0301-2115(00)00564-9