NMR local range investigations in amorphous starchy substrates I. Structural heterogeneity probed by (13)C CP-MAS NMR

The (13)C CP-MAS (Cross Polarization and Magic Angle Spinning) NMR signatures of a series of amorphous and semi-crystalline samples prepared from various starchy substrates (native potato starch, amylopectin, amylose) following different techniques of preparation (casting, freeze drying, solvent exc...

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Bibliographic Details
Published in:International journal of biological macromolecules 2001-08, Vol.29 (2), p.127-136
Main Authors: Paris, M, Bizot, H, Emery, J, Buzaré, J Y, Buléon, A
Format: Article
Language:English
Subjects:
Amylopectin - chemistry
Amylose - chemistry
Carbohydrate Conformation
Carbon Isotopes
Crystallography, X-Ray
Macromolecular Substances
Magnetic Resonance Spectroscopy - methods
Molecular Structure
Solanum tuberosum - chemistry
Starch - chemistry
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Summary:The (13)C CP-MAS (Cross Polarization and Magic Angle Spinning) NMR signatures of a series of amorphous and semi-crystalline samples prepared from various starchy substrates (native potato starch, amylopectin, amylose) following different techniques of preparation (casting, freeze drying, solvent exchange) are compared. Decompositions of the C1 resonance spectra reveal the existence of four or five main types of alpha(1-4) linkages, which can be quantified. The influence of the intrinsic primary structure (linear or branched) and of the preparation procedure on conformational changes and resulting crystallinity are interpreted in terms of distributions of average glycosidic linkages dihedral angles (Phi, Psi). The role of hydration is also considered. An improved understanding at different structural levels is obtained in relation to local and intermediate range orders. Such information may be useful for the understanding of the structural evolution of a large variety of starchy substrates before or after treatments widely used in industrial processes.
ISSN:0141-8130
DOI:10.1016/S0141-8130(01)00160-X