The Role of Stem Cells in Midgut Growth and Regeneration

The Manduca sexta (L.) [Lepidoptera: Sphingidae] and Heliothis virescens (F.) [Lepidoptera: Noctuidae] midguts consist of a pseudostratified epithelium surrounded by striated muscle and tracheae. This epithelium contains goblet, columnar, and basal stem cells. The stem cells are critically important...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Animal 2001-06, Vol.37 (6), p.338-342
Main Authors: Hakim, Raziel S., Baldwin, Kate M., Marcia Loeb
Format: Article
Language:English
Subjects:
Animals
Cellular differentiation
Cultured cells
Digestive System - cytology
Digestive System - growth & development
Diploidy
Goblets
Insect larvae
Instars
Intestines
Manduca - cytology
Manduca - physiology
Mesenchymal stem cells
Midgut
Moths - cytology
Moths - physiology
Regeneration - physiology
Stem cells
Stem Cells - cytology
Stem Cells - physiology
Symposium on Insect Midgut
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Summary:The Manduca sexta (L.) [Lepidoptera: Sphingidae] and Heliothis virescens (F.) [Lepidoptera: Noctuidae] midguts consist of a pseudostratified epithelium surrounded by striated muscle and tracheae. This epithelium contains goblet, columnar, and basal stem cells. The stem cells are critically important in that they are capable of massive proliferation and differentiation. This growth results in a fourfold enlargement of the midgut at each larval molt. The stem cells are also responsible for limited cell replacement during repair. While the characteristics of the stem cell population vary over the course of an instar, stem cells collected early in an instar and those collected late can start in vitro cultures. Cultures of larval stem, goblet, and columnar cells survive in vitro for several mo through proliferation and differentiation of the stem cells. One of the two polypeptide differentiation factors which have been identified and characterized from the culture medium has now been shown to be present in midgut in vivo. Thus the ability to examine lepidopteran midgut stem cell growth in vitro and in vivo is proving to be effective in determining the basic features of stem cell action and regulation.
ISSN:1071-2690
1543-706X
DOI:10.1007/bf02577567