Structural characteristics and properties of the regenerated silk fibroin prepared from formic acid

Structural characteristics and thermal and solution properties of the regenerated silk fibroin (SF) prepared from formic acid (FU) were compared with those of SF from water (AU). According to the turbidity and shear viscosity measurement, SF formic acid solution was stable and transparent, no molecu...

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Bibliographic Details
Published in:International journal of biological macromolecules 2001-08, Vol.29 (2), p.91-97
Main Authors: Um, In Chul, Kweon, HaeYong, Park, Young Hwan, Hudson, Sam
Format: Article
Language:English
Subjects:
Animals
Bombyx - chemistry
Calorimetry, Differential Scanning
Crystallization
Fibroins - chemistry
Fibroins - isolation & purification
Formates
Formic acid
Insect Proteins - chemistry
Insect Proteins - isolation & purification
Macromolecular Substances
Molecular Structure
Nephelometry and Turbidimetry
Protein Structure, Secondary
Rheology
Silk
Silk fibroin
Solutions
Spectroscopy, Fourier Transform Infrared
Thermodynamics
Viscosity
Water
X-Ray Diffraction
β-sheet structure
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Summary:Structural characteristics and thermal and solution properties of the regenerated silk fibroin (SF) prepared from formic acid (FU) were compared with those of SF from water (AU). According to the turbidity and shear viscosity measurement, SF formic acid solution was stable and transparent, no molecular aggregations occurred. The sample FU exhibited the β-sheet structure, while AU random coil conformation using Fourier transform infrared (FTIR), X-ray diffraction (XRD), and differential scanning calorimetry. The effects of methanol treatment on samples were also examined. According to the measurement of crystallinity (XRD) and crystallinity index (FTIR), the concept of long/short-range ordered structure formation was proposed. Long-range ordered crystallites are predominantly formed for methanol treated SF film while SF film cast from formic acid favors the formation of short-range ordered structure. The relaxation temperatures of SF films measured by dynamic thermomechanical analysis supported the above mechanism due to the sensitivity of relaxation temperature on the short-range order.
ISSN:0141-8130
1879-0003
DOI:10.1016/S0141-8130(01)00159-3