Hrs-2 Regulates Receptor-mediated Endocytosis via Interactions with Eps15

Hrs-2, via interactions with SNAP-25, plays a regulatory role on the exocytic machinery. We now show that Hrs-2 physically interacts with Eps15, a protein required for receptor-mediated endocytosis. The Hrs-2/Eps15 interaction is calcium dependent, inhibited by SNAP-25 and α-adaptin, and results in...

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Bibliographic Details
Published in:The Journal of biological chemistry 2000-05, Vol.275 (20), p.15271-15278
Main Authors: Bean, Andrew J., Davanger, Svend, Chou, Marian F., Gerhardt, Brenda, Tsujimoto, Susan, Chang, YuChieh
Format: Article
Language:English
Subjects:
Adaptor Proteins, Signal Transducing
Adenosine Triphosphatases - analysis
Adenosine Triphosphatases - metabolism
Animals
Brain - metabolism
Calcium - metabolism
Calcium-Binding Proteins - metabolism
Cerebellum - metabolism
Endocytosis - physiology
Endosomal Sorting Complexes Required for Transport
Endosomes - metabolism
Endosomes - ultrastructure
HeLa Cells
Humans
Intracellular Signaling Peptides and Proteins
Kinetics
Membrane Proteins
Microscopy, Immunoelectron
Nerve Tissue Proteins - metabolism
Neurons - metabolism
Neurons - ultrastructure
Phosphoproteins - metabolism
Rats
Receptors, Cell Surface - physiology
Recombinant Proteins - metabolism
Signal Transduction
Synaptosomal-Associated Protein 25
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Summary:Hrs-2, via interactions with SNAP-25, plays a regulatory role on the exocytic machinery. We now show that Hrs-2 physically interacts with Eps15, a protein required for receptor-mediated endocytosis. The Hrs-2/Eps15 interaction is calcium dependent, inhibited by SNAP-25 and α-adaptin, and results in the inhibition of receptor-mediated endocytosis. Immunoelectron microscopy reveals Hrs-2 localization on the limiting membrane of multivesicular bodies, organelles in the endosomal pathway. These data show that Hrs-2 regulates endocytosis, delineate a biochemical pathway (Hrs-2-Eps15-AP2) in which Hrs-2 functions, and suggest that Hrs-2 acts to provide communication between endo- and exocytic processes.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.275.20.15271