Increase in Lipid Content in Potato Tubers Modified by 14-3-3 Gene Overexpression

Recently, transgenic potato plants were created with overexpression of the 14-3-3 protein derived from Cucurbita pepo. Detailed analysis of those plants suggested that the function of the isolated 14-3-3 isoform is in the control of carbohydrate and lipid metabolism in the plants. 14-3-3 protein ove...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2001-08, Vol.49 (8), p.3638-3643
Main Authors: Prescha, Anna, Świȩdrych, Anna, Biernat, Jadwiga, Szopa, Jan
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Chemical constitution
Economic plant physiology
Fundamental and applied biological sciences. Psychology
Gene Expression
Genetic engineering applications
Genetics and breeding of economic plants
Linoleic Acid - analysis
Lipids - analysis
Plant breeding: fundamental aspects and methodology
Plants, Genetically Modified
Solanum tuberosum - chemistry
Solanum tuberosum - genetics
Solanum tuberosum - metabolism
Transgenes
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Summary:Recently, transgenic potato plants were created with overexpression of the 14-3-3 protein derived from Cucurbita pepo. Detailed analysis of those plants suggested that the function of the isolated 14-3-3 isoform is in the control of carbohydrate and lipid metabolism in the plants. 14-3-3 protein overexpression gave rise to an increase in soluble sugar and catecholamine contents in both leaves and tubers. It is proposed that 14-3-3 protein affects carbohydrate metabolism in potato plants via regulation of catecholamine synthesis. Furthermore, genetically modified potato tubers with 14-3-3 protein overexpression showed changes in lipid content and composition. The transgenic potato tubers contained 69% more total fat compared to the wild-type plant. Separation of tuber lipids into polar and nonpolar fractions revealed that the transgenic potato tubers contained almost 3 times more nonpolar lipids than the control. Analysis of fatty acid composition, conducted by the means of gas chromatography, showed that linoleic acid was the main fatty acid present in the tubers of both modified and control potato plants. In the nonpolar fraction of the fat of the transgenic tubers the unsaturated fatty acids exhibited a higher participation in the sum of all fatty acids. Keywords: 14-3-3 protein; transgenic plant; catecholamines; carbohydrate metabolism; lipid analysis; Solanum tuberosum
ISSN:0021-8561
1520-5118
DOI:10.1021/jf010258o