Pantothenate synthetase from Fusarium oxysporum f. sp. lycopersici is induced by alpha-tomatine

The steroidal glycoalkaloid alpha-tomatine which is present in tomato (Lycopersicum sculentum) is assumed to protect the plant against phytopathogenic fungi. We have isolated a gene from the fungal pathogen Fusarium oxysporum f. sp. lycopersici that is induced by this glycoalkaloid. This gene, desig...

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Bibliographic Details
Published in:Molecular genetics and genomics : MGG 2001-07, Vol.265 (5), p.922-929
Main Authors: Perez Espinosa, A, Roldan Arjona, T, Ruiz Rubio, M
Format: Article
Language:English
Subjects:
a-tomatine
Amino Acid Sequence
Antifungal Agents - pharmacology
Base Sequence
Cloning, Molecular
Fusarium - genetics
Fusarium lycopersici
Fusarium oxysporum
Gene Expression Regulation, Fungal - drug effects
Molecular Sequence Data
panC gene
Peptide Synthases - genetics
Sequence Alignment
Tomatine - pharmacology
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Summary:The steroidal glycoalkaloid alpha-tomatine which is present in tomato (Lycopersicum sculentum) is assumed to protect the plant against phytopathogenic fungi. We have isolated a gene from the fungal pathogen Fusarium oxysporum f. sp. lycopersici that is induced by this glycoalkaloid. This gene, designated panC, encodes a predicted protein with a molecular mass of 41 kDa that shows a high degree of sequence similarity to pantothenate synthetases from yeast, plants and bacteria. Recombinant PanC protein from F. oxysporum has been over-expressed in Escherichia coli and purified to homogeneity. It shows pantothenate synthetase activity in the presence of D-pantoate, beta-alanine and ATP. The panC gene from F. oxysporum functionally complements an E. coli panC mutant, demonstrating that the PanC protein functions in vivo as a pantothenate synthetase. Southern analysis of F. oxysporum genomic DNA from other formae speciales indicates that there is a single copy of the pantothenate syntethase gene in this fungus. The presence of a STRE consensus sequence (CCCCT) in the promoter region of the gene suggests that the induction of panC may be part of a cellular stress response triggered by alpha-tomatine.
ISSN:1617-4615
1617-4623
DOI:10.1007/s004380100491