An improved method for the rapid assessment of persisting chylomicron remnant concentrations

Persisting chylomicron remnant concentrations have been linked to premature atherosclerosis. The analysis of persisting chylomicron remnant concentrations by an oral triglyceride tolerance test, however, is time-consuming for the study subjects and requires large resources in the laboratory. Therefo...

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Bibliographic Details
Published in:Clinical biochemistry 2001-07, Vol.34 (5), p.387-394
Main Authors: Orth, Matthias, Weinland-Wilken, Christian, Westphal, Sabine, Luley, Claus
Format: Article
Language:English
Subjects:
Adult
Aged
Apolipoprotein E2
Apolipoprotein E3
Apolipoproteins E - analysis
Chromatography, Gel
Chylomicron Remnants
Chylomicrons - analysis
Dietary Fats - blood
Electrophoresis, Polyacrylamide Gel
Female
Homozygote
Humans
Hyperlipoproteinemia Type III - blood
Immunoblotting
Lipoproteins - blood
Male
Middle Aged
Phenotype
Postprandial
Postprandial Period - physiology
Reproducibility of Results
Retinyl palmitate
Sensitivity and Specificity
Spectrometry, Fluorescence
Standardized testing
Surveys and Questionnaires
Ultracentrifugation
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Summary:Persisting chylomicron remnant concentrations have been linked to premature atherosclerosis. The analysis of persisting chylomicron remnant concentrations by an oral triglyceride tolerance test, however, is time-consuming for the study subjects and requires large resources in the laboratory. Therefore, only small numbers of subjects have been studied in the past. We describe major improvements of the testing procedure in regard of composition of the fatty meal, of patient testing, and measurement of postprandial remnants. Shifting the time of the (ready-to-use) fatty drink from the morning hours to bedtime was well accepted by the study subjects and allowed the analysis of blood samples drawn at the morning with minimal impact on the participants’ time and with minimal interferences by confounding factors ( e.g. smoking, additional food intake, physical activity). Chylomicron remnants were measured by fluorometry of the supernatant after ultracentrifugation. This procedure was sensitive, was specific for chylomicron remnants, and was easy to perform. The biological validity of the improved procedure was evaluated by studying type III hyperlipoproteinemia patients and normolipemic apolipoprotein (Apo) E2 homozygotes. In conclusion, this improved test permits the rapid testing for persisting chylomicron remnants in the clinical routine and in large epidemiological studies.
ISSN:0009-9120
1873-2933
DOI:10.1016/S0009-9120(01)00233-8