Nuclear bodies in the oocyte nucleus of ground beetles are enriched in snRNPs

Within the oocyte nucleus of many insect species, a variable number of intensely stained spherical bodies occur. These nuclear bodies differ significantly from nucleoli and their precise role in nuclei has not been elucidated yet. I have examined some of the histochemical properties as well as the m...

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Bibliographic Details
Published in:Tissue & cell 2001-08, Vol.33 (4), p.395-401
Main Author: Jaglarz, M.K.
Format: Article
Language:English
Subjects:
Animals
Cajal bodies, snRNPs, germinal vesicle, oocyte, ground beetles
Carabidae
Cell Nucleus - chemistry
Cell Nucleus - ultrastructure
Coleoptera - chemistry
Coleoptera - ultrastructure
Female
Immunohistochemistry
Microscopy, Immunoelectron
nuclear bodies
Nucleolus Organizer Region - metabolism
Oocytes - chemistry
Oocytes - ultrastructure
Ovary - cytology
Ovary - ultrastructure
Ribonucleoproteins, Small Nuclear - analysis
Ribonucleoproteins, Small Nuclear - ultrastructure
Silver Staining
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Summary:Within the oocyte nucleus of many insect species, a variable number of intensely stained spherical bodies occur. These nuclear bodies differ significantly from nucleoli and their precise role in nuclei has not been elucidated yet. I have examined some of the histochemical properties as well as the molecular composition of these structures in a representative of ground (carabid) beetles. I demonstrate, using molecular markers, that the nuclear bodies are composed of small nuclear RNAs and associated proteins, including p80 coilin. Hence, they correspond to Cajal bodies (= coiled bodies) described in somatic cell nuclei as well as oocyte germinal vesicles in plant and animal organisms. It is suggested that Cajal bodies in the carabid germinal vesicle serve as a storage site for splicing factors.
ISSN:0040-8166
1532-3072
DOI:10.1054/tice.2001.0193