Separation of polymerase chain reaction amplified bird genes by capillary electrophoresis

Female birds possess one Z and one W chromosome, whereas male birds possess two identical Z chromosomes. Thus, the presence of a W genetic marker is diagnostic of the female sex. Capillary electrophoresis with buffer containing an entangled solution of hydroxyethylcellulose was used to separate the...

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Bibliographic Details
Published in:Electrophoresis 2000-04, Vol.21 (7), p.1336-1340
Main Authors: Lesaicherre, Marie-Laure, Li, Sam F. Y., Lee, Hian K.
Format: Article
Language:English
Subjects:
Animals
Benzothiazoles
Bird sexing genes
Birds - genetics
Capillary electrophoresis
DNA-Binding Proteins - genetics
Electrophoresis, Capillary - methods
Female
Fluorescent Dyes
Genes
Male
Polymerase chain reaction
Polymerase Chain Reaction - methods
Quinolines
Thiazoles
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Summary:Female birds possess one Z and one W chromosome, whereas male birds possess two identical Z chromosomes. Thus, the presence of a W genetic marker is diagnostic of the female sex. Capillary electrophoresis with buffer containing an entangled solution of hydroxyethylcellulose was used to separate the polymerase chain reaction (PCR) amplified bird sexing genes CHD‐Z and CHD‐W. The relative standard deviations (RSD) were less than 0.6% for the male genes and less than 0.4% for the female genes for six runs and detection limits of 0.1 ng/μL were obtained with laser‐induced fluorescence (LIF) detection. Using a DNA ladder and theoretical models for DNA separation in sieving media, the sizes of the two bird genes were determined.
ISSN:0173-0835
1522-2683
DOI:10.1002/(SICI)1522-2683(20000401)21:7<1336::AID-ELPS1336>3.0.CO;2-I