Cloning and Expression of the Platelet-Specific Collagen Receptor Glycoprotein VI

• Published in: Thrombosis research 2000-05, Vol.98 (4), p.301-309
• Main Authors: Miura, Yoshiki, Ohnuma, Masaaki, Jung, Stephanie M, Moroi, Masaaki
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Base Sequence; Biological and medical sciences; Blood coagulation. Blood cells; Blood Platelets - metabolism; cDNA Cloning; Chromatography, Affinity; Cloning, Molecular; Collagen; Collagen - metabolism; Crotalid Venoms - metabolism; DNA, Complementary - genetics; Expression; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Leukemic; General aspects, investigation methods, hemostasis, fibrinolysis; Genes, Immunoglobulin; GPVI; Ig-like receptor; Lectins, C-Type; Leukemia, Megakaryoblastic, Acute - genetics; Leukemia, Megakaryoblastic, Acute - metabolism; Leukemia, Megakaryoblastic, Acute - pathology; Molecular and cellular biology; Molecular Sequence Data; Multigene Family; Neoplasm Proteins - biosynthesis; Neoplasm Proteins - genetics; Open Reading Frames - genetics; Platelet; Platelet Membrane Glycoproteins - biosynthesis; Platelet Membrane Glycoproteins - genetics; Platelet Membrane Glycoproteins - isolation & purification; Polymerase Chain Reaction; Protein Structure, Tertiary - genetics; Receptors, Immunologic - chemistry; Receptors, KIR; Recombinant Fusion Proteins - biosynthesis; RNA, Messenger - genetics; RNA, Messenger - isolation & purification; Sequence Alignment; Sequence Homology, Amino Acid; Transfection; Tumor Cells, Cultured
• ISSN: 0049-3848; 1879-2472
• DOI: 10.1016/S0049-3848(00)00182-1

Platelet glycoprotein VI (GP VI) was purified from platelet membranes and its internal amino acid sequences were determined. The cloned cDNA of GP VI indicates an open reading frame coding for 20 amino acid signal sequences and a mature protein of 319 amino acids. Its extracellular region has two Ig-like domains and a mucin-like, Ser/Thr-rich region, suggesting that GP VI is a member of the paired Ig-like receptor family. GP VI-transfected cells contained convulxin-(reactive) and antibody against recombinant GP VI-reactive protein bands that migrated at the same position as platelet GP VI in SDS/PAGE-electroblotting. These data indicate that the protein deduced from the cloned cDNA corresponds to platelet GP VI.