New pseudo-stationary phases for electrokinetic capillary chromatography: Complexes between bovine serum albumin and sodium dodecyl sulfate

The complexes formed between a protein (bovine serum albumin, BSA) and a surfactant (sodium dodecyl sulfate, SDS) were studied as separation carriers in electrokinetic chromatography. Selectivities different from those with either SDS or BSA alone in the background electrolyte (BGE) were obtained. S...

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Bibliographic Details
Published in:Journal of Chromatography A 2000-04, Vol.876 (1), p.169-182
Main Authors: Gaillon, L, Cozette, S, Lelievre, J, Gaboriaud, R
Format: Article
Language:English
Subjects:
Albumin
Analytical chemistry
Animals
Cattle
Chemistry
Chlorophenols
Chromatographic methods and physical methods associated with chromatography
Chromatography, Micellar Electrokinetic Capillary - methods
Electrolytes - chemistry
Exact sciences and technology
Nitrophenols
Other chromatographic methods
Phenols
Serum Albumin, Bovine - chemistry
Sodium dodecyl sulfate
Sodium Dodecyl Sulfate - chemistry
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Summary:The complexes formed between a protein (bovine serum albumin, BSA) and a surfactant (sodium dodecyl sulfate, SDS) were studied as separation carriers in electrokinetic chromatography. Selectivities different from those with either SDS or BSA alone in the background electrolyte (BGE) were obtained. Separation performances were demonstrated to be closely related to the type of complex formed, as predicted by the isotherm curve of SDS on BSA. For each composition of background electrolyte, capacity factors and resolutions were calculated. We compared the results with these complexes to electropherograms using BGE containing either BSA or SDS alone. The separation of a mixture of phenols indicate that some compositions of the BSA–SDS complexes are efficient selectors.
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)00234-X