Modulation of c-jun and c-fos Transcription by UVB and UVA Radiations in Human Dermal Fibroblasts and KB Cells

We have previously demonstrated that the oxidizing component of ultraviolet-A (UVA) plays a central role in the activation of the nuclear oncogene and transcription factor, c-fos, in cultured human skin fibroblasts. We have now shown that expression of both c-jun and c-fos (AP-1) family of transcrip...

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Bibliographic Details
Published in:Photochemistry and photobiology 2000-05, Vol.71 (5), p.551-558
Main Authors: Soriani, Marco, Hejmadi, Vidya, Tyrrell, Rex M.
Format: Article
Language:English
Subjects:
Cell Line
ENVIRONMENTAL PHOTOBIOLOGY AND UVR EFFECTS
Fibroblasts - cytology
Fibroblasts - metabolism
Fibroblasts - radiation effects
Gene Expression Regulation - radiation effects
Genes, fos
Genes, jun
Humans
Skin - cytology
Skin - metabolism
Skin - radiation effects
Transcription, Genetic - radiation effects
Ultraviolet Rays
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Summary:We have previously demonstrated that the oxidizing component of ultraviolet-A (UVA) plays a central role in the activation of the nuclear oncogene and transcription factor, c-fos, in cultured human skin fibroblasts. We have now shown that expression of both c-jun and c-fos (AP-1) family of transcription factors is modulated by short and long wavelength solar ultraviolet (UV) radiation in human fibroblasts and human KB cells. UVA radiation activated c-jun and c-fos in both fibroblasts and KB cells, whereas ultraviolet-B (UVB) radiation activates such oncogenes only in KB cells. Moreover, decreasing the intracellular levels of reducing equivalents in human fibroblasts by glutathione (GSH) depletion lowered the UVA dose threshold for c-jun and c-fos activation several-fold and greatly amplified the UVA-mediated activation of such genes. A more modest effect was observed in GSH-depleted KB cells. In both GSH-depleted fibroblasts and KB cells, UVB radiation failed to amplify c-jun and c-fos activation indicating that the oxidative component of UVB plays a minor role in the modulation of such oncogene expression. These findings clearly indicate that both c-jun and c-fos are activated by the oxidizing component of UVA radiation in human fibroblasts and KB cells, while UVB-mediated modulation seems to be restricted to human epithelial cells and does not involve oxidizing intermediates.
ISSN:0031-8655
1751-1097
DOI:10.1562/0031-8655(2000)071<0551:MOCJAC>2.0.CO;2