Inactivation of Orange Pectinesterase by Combined High-Pressure and -Temperature Treatments:  A Kinetic Study

• Published in: Journal of agricultural and food chemistry 2000-05, Vol.48 (5), p.1960-1970
• Main Authors: Van den Broeck, Ilse, Ludikhuyze, Linda R, Van Loey, Ann M, Hendrickx, Marc E
• Format: Article
• Language: English
• Subjects: Carboxylic Ester Hydrolases - antagonists & inhibitors; Carboxylic Ester Hydrolases - metabolism; Citrus - enzymology; Kinetics; Pressure; Temperature
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf990659s

Pressure and/or temperature inactivation of orange pectinesterase (PE) was investigated. Thermal inactivation showed a biphasic behavior, indicating the presence of labile and stable fractions of the enzyme. In a first part, the inactivation of the labile fraction was studied in detail. The combined pressure−temperature inactivation of the labile fraction was studied in the pressure range 0.1−900 MPa combined with temperatures from 15 to 65 °C. Inactivation in the pressure−temperature domain specified could be accurately described by a first-order fractional conversion model, estimating the inactivation rate constant of the labile fraction and the remaining activity of the stable fraction. Pressure and temperature dependence of the inactivation rate constants of the labile fraction was quantified using the Eyring and Arrhenius relations, respectively. By replacing in the latter equation the pressure-dependent parameters (E a, k ref T ) by mathematical expressions, a global model was formulated. This mathematical model could accurately predict the inactivation rate constant of the labile fraction of orange PE as a function of pressure and temperature. In a second part, the stable fraction was studied in more detail. The stable fraction inactivated at temperatures exceeding 75 °C. Acidification (pH 3.7) enhanced thermal inactivation of the stable fraction, whereas addition of Ca2+ ions (1 M) suppressed inactivation. At elevated pressure (up to 900 MPa), an antagonistic effect of pressure and temperature on the inactivation of the stable fraction was observed. The antagonistic effect was more pronounced in the presence of a 1 M CaCl2 solution as compared to the inactivation in water, whereas it was less pronounced for the inactivation in acid medium. Keywords: Orange pectinesterase; thermal stability; pressure stability; kinetics; stable fraction