Immunomagnetic selection of CD34+ cells: factors influencing component purity and yield

• Published in: Transfusion (Philadelphia, Pa.) Pa.), 2000-05, Vol.40 (5), p.507-512
• Main Authors: Hildebrandt, M., Serke, S., Meyer, O., Ebell, W., Salama, A.
• Format: Article
• Language: English
• Subjects: ALL = acute lymphatic leukemia; Antigens, CD34 - blood; Hematocrit; Humans; Immunomagnetic Separation - methods; Linear Models; NC(s) = nucleated cell(s); Neuroblastoma - blood; Precursor Cell Lymphoblastic Leukemia-Lymphoma - blood; Sarcoma, Ewing - blood
• ISSN: 0041-1132; 1537-2995
• DOI: 10.1046/j.1537-2995.2000.40050507.x

BACKGROUND: In immunomagnetic selection of CD34+ cells from HPC transplants, not all factors that affect yield and purity of CD34+ cells are known. METHODS: Forty‐three consecutive procedures of immunomagnetic selection of CD34+ cells from peripheral blood HPCs and bone marrow harvests (autologous harvests, n = 27; allogeneic harvests; n=16) were performed by use of a cell selection system (Isolex 300i, Baxter Immunotherapy). The composition of the starting component and the subsets of CD34+ cells were analyzed for correlation with the yield and purity of the final component. RESULTS: The mean purity of the final components was 84.3 percent (range, 27‐99%), and the mean yield was 51.4 percent (range, 9.4‐80.4%). Partial regression analysis showed that, among the factors correlating with purity and/or yield, the RBC volume in the starting fraction had the highest predictive impact on the purity and yield of CD34+ cells, even after the exclusion of procedures using bone marrow harvests as an HPC source (beta coefficient, –0.704; p = 0.001). CONCLUSION: The use of the Isolex 300i system allows efficient recovery of CD34+ cells in routine selection procedures. The volume of RBCs in the starting component should be minimized to ensure a high yield and purity of the final component.