Effect of peroxynitrite on glutaredoxin

Glutaredoxin is an important enzyme in thiol homeostasis. As a thioltransferase, it reduces oxidized thiols. It also has dehydroascorbate reductase (DHAR) activity to reduce dehydroascorbate (DHA) to ascorbic acid. Peroxynitrite (ONOO-) is one of the most active elements of oxidative stress that can...

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Bibliographic Details
Published in:Human & experimental toxicology 2001-07, Vol.20 (7), p.373-376
Main Authors: Aykaç-Toker, G, Bulgurcuogélu, S, Koçak-Toker, N
Format: Article
Language:English
Subjects:
Acids
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Cell Culture Techniques
dehydroascorbate reductase
Enzymes
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
glutaredoxin
Glutaredoxins
Homeostasis
Liver - drug effects
Male
Nitric oxide
Oxidative stress
Oxidoreductases - drug effects
Oxidoreductases - metabolism
Peroxynitrous Acid - pharmacology
Potassium
Proteins - drug effects
Proteins - metabolism
Rats
Rats, Wistar
thioltransferase
Toxicology
Transferases
Variance analysis
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Summary:Glutaredoxin is an important enzyme in thiol homeostasis. As a thioltransferase, it reduces oxidized thiols. It also has dehydroascorbate reductase (DHAR) activity to reduce dehydroascorbate (DHA) to ascorbic acid. Peroxynitrite (ONOO-) is one of the most active elements of oxidative stress that can be formed wherever nitric oxide and superoxide are produced simultaneously. ONOO- is known to react with free thiols easily. To observe the effect of ONOO- on glutaredoxin, rat liver cytosolic fractions were incubated with 0–250 MONOO -. Thioltransferase activity was found to be decreased as ONOO- concentration increased. The inhibition was not reversible with dithiothreitol (DTT). In cytosol besides glutaredoxin, another enzyme with DHAR activity is also present. In our study, the cytosolic DHAR activity which consisted both enzymes, was also inhibited by ONOO-, but DTT was able to return the activity almost completely.
ISSN:0960-3271
1477-0903
DOI:10.1191/096032701680350578