Intranasal immunization with protein-linked phosphorylcholine protects mice against a lethal intranasal challenge with Streptococcus pneumoniae

• Published in: Vaccine 2000-07, Vol.18 (26), p.2991-2998
• Main Authors: Trolle, Sylvine, Chachaty, Elisabeth, Kassis-Chikhani, Najiby, Wang, Chuansheng, Fattal, Elias, Couvreur, Patrick, Diamond, Betty, Alonso, Jean-Michel, Andremont, Antoine
• Format: Article
• Language: English
• Subjects: Administration, Intranasal; Animals; Antigens; Bacteriology; Biological and medical sciences; Deoxyribonucleic acid; DNA; Entrapment; Female; Fluids; Fundamental and applied biological sciences. Psychology; Gram-positive bacteria; Immunity, Mucosal; Immunization; Immunoglobulin A; Immunoglobulin A - analysis; Immunoglobulin G; Immunoglobulin G - analysis; Immunoglobulins; Lung - microbiology; Lungs; Mice; Mice, Inbred BALB C; Microbiology; Microspheres; Mouse devices; Mucosa; Nasal immunization; Phosphorylcholine; Phosphorylcholine - administration & dosage; Phosphorylcholine - immunology; Pneumococcal Infections - mortality; Pneumococcal Infections - prevention & control; Pneumonia; Proteins; Salmonella; Secretions; Streptococcus infections; Streptococcus pneumoniae; Survival; Vaccination; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/S0264-410X(00)00089-X

Immunization against phosphorylcholine (PC) linked to a protein protects mice against Streptococcus pneumoniae when used parenterally, and against Salmonella typhimurium when used orally after entrapment in d,l-Lactide-co-Glycolide microspheres. Here, we immunized BALB/c mice intranasally with a serotype 3 S. pneumoniae strain. Immunization was followed by a rise in anti-PC IgA and IgG titers in serum and in pulmonary secretions, but not by any rise in anti ds-DNA antibody nor any glomerular Ig deposition. The survival rates were 91 and 76% in the two groups of mice, respectively. These rates were significantly higher than those in control mice immunized intranasally either with Thyr loaded in microspheres (0%), blank microspheres (22%), free Thyr (17%), and saline (18%). This demonstrates that the mucosal route is effective for vaccination against S. pneumoniae pneumonia with PC linked to a protein carrier. It constitutes another important step forward in the development of the concept that PC can be used as a mucosal immunogen for protection against the different diseases caused by PC-bearing bacteria.