Effects of Buddleja globosa leaf and its constituents relevant to wound healing

• Published in: Journal of ethnopharmacology 2001-10, Vol.77 (2), p.219-226
• Main Authors: Mensah, A.Y., Sampson, J., Houghton, P.J., Hylands, P.J., Westbrook, J., Dunn, M., Hughes, M.A., Cherry, G.W.
• Format: Article
• Language: English
• Subjects: Antioxidants - isolation & purification; Antioxidants - therapeutic use; Bioassays; Biological and medical sciences; Buddleja globosa; Cells, Cultured; Chile; Dose-Response Relationship, Drug; Fibroblasts - drug effects; Flavonoids; General pharmacology; Humans; Hydrogen Peroxide - antagonists & inhibitors; Medical sciences; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Phenylethanoids; Phytotherapy; Plant Extracts - isolation & purification; Plant Extracts - therapeutic use; Plant Leaves; Wound Healing - drug effects; Wound-healing
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/S0378-8741(01)00297-5

An aqueous extract of Buddleja globosa leaves, used traditionally in Chile for wound healing, was tested for the ability to stimulate growth of fibroblasts in vitro and for antioxidant activity in the same fibroblast cell system challenged with hydrogen peroxide. Low concentrations of the extract gave an increase in fibroblast growth which was not statistically significant but cytotoxicity was observed at concentrations greater than 50 μg/ml. The extract showed strong antioxidant effect and fractionation led to the isolation of three flavonoids and two caffeic acid derivatives, each of which was shown to contribute to the antioxidant effect at concentrations below 10 μg/ml. These activities would accelerate the healing of wounds.