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Differential expression of amiloride-sensitive NA + channel subunits messenger RNA in the rat uterus

The expression of the amiloride-sensitive Na + channel was investigated by reverse transcription-polymerase chain reaction (RT-PCR) in uteri from pregnant and non-pregnant rats. Three subunits (α,β,γ) of this epithelial Na + channel have been characterized in the rat. All three subunit mRNAs were pr...

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Published in:	Life sciences (1973) 2000-04, Vol.66 (22), p.PL313-PL317
Main Authors:	Pinto, Francisco M., Cintado, Cristina G., Mérida, Angel, Hidalgo, Agustin, Candenas, M.Luz
Format:	Article
Language:	English
Subjects:	Amiloride - pharmacology Animals Diuretics - pharmacology epithelial Na + channel Epithelial Sodium Channels Female Gene Expression Regulation mRNA expression polymerase chain reaction Pregnancy rat uterus Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Sodium Channels - genetics Sodium Channels - metabolism Uterus - metabolism
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cited_by	cdi_FETCH-LOGICAL-c361t-10e6e0a5029b2766f8acd20a28f454aee423a81b1be821a029aeb5f229b95cc43
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container_end_page	PL317
container_issue	22
container_start_page	PL313
container_title	Life sciences (1973)
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creator	Pinto, Francisco M. Cintado, Cristina G. Mérida, Angel Hidalgo, Agustin Candenas, M.Luz
description	The expression of the amiloride-sensitive Na + channel was investigated by reverse transcription-polymerase chain reaction (RT-PCR) in uteri from pregnant and non-pregnant rats. Three subunits (α,β,γ) of this epithelial Na + channel have been characterized in the rat. All three subunit mRNAs were present in day 16 pregnant uteri while only α and β subunit mRNAs were detected in uteri from non-pregnant or day 1 postpartum rats. The level of expression of the α subunit was similar in day 16 pregnant and non-pregnant animals while the level of expression of the β subunit was higher in pregnant than in non-pregnant rats. These findings show that amiloride-sensitive Na + channels are expressed in the rat uterus and that mRNA expression levels of the α, β and γ subunits are selectively and differentially regulated during pregnancy in the rat.
doi_str_mv	10.1016/S0024-3205(00)00550-6
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Three subunits (α,β,γ) of this epithelial Na + channel have been characterized in the rat. All three subunit mRNAs were present in day 16 pregnant uteri while only α and β subunit mRNAs were detected in uteri from non-pregnant or day 1 postpartum rats. The level of expression of the α subunit was similar in day 16 pregnant and non-pregnant animals while the level of expression of the β subunit was higher in pregnant than in non-pregnant rats. 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These findings show that amiloride-sensitive Na + channels are expressed in the rat uterus and that mRNA expression levels of the α, β and γ subunits are selectively and differentially regulated during pregnancy in the rat.</description><subject>Amiloride - pharmacology</subject><subject>Animals</subject><subject>Diuretics - pharmacology</subject><subject>epithelial Na + channel</subject><subject>Epithelial Sodium Channels</subject><subject>Female</subject><subject>Gene Expression Regulation</subject><subject>mRNA expression</subject><subject>polymerase chain reaction</subject><subject>Pregnancy</subject><subject>rat uterus</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Sodium Channels - genetics</subject><subject>Sodium Channels - metabolism</subject><subject>Uterus - metabolism</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNqF0EtLxDAQB_AgiruufgQlJ1GkOkn62pMs6xMWBR_nkKZTjbTpmqSLfnu7D8Sbp7n8_jPMn5BDBucMWHrxDMDjSHBITgBOAZIEonSLDFmejSNIBdsmw18yIHvef8BSZWKXDBjkIhaQDUl5ZaoKHdpgVE3xa-7Qe9Na2lZUNaZunSkx8mi9CWaB9GFCz6h-V9ZiTX1XdNYET5s-hPYNHX3qgbE0vCN1KtAuoOv8PtmpVO3xYDNH5PXm-mV6F80eb--nk1mkRcpCxABTBJUAHxc8S9MqV7rkoHhexUmsEGMuVM4KVmDOmeqZwiKpeM_HidaxGJHj9d65az879EE2xmusa2Wx7bzMGIszlokeJmuoXeu9w0rOnWmU-5YM5LJeuapXLruTAHJVr0z73NHmQFc0WP5JrfvsweUaYP_mwqCTXhu0GkvjUAdZtuafEz9ygYov</recordid><startdate>20000421</startdate><enddate>20000421</enddate><creator>Pinto, Francisco M.</creator><creator>Cintado, Cristina G.</creator><creator>Mérida, Angel</creator><creator>Hidalgo, Agustin</creator><creator>Candenas, M.Luz</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000421</creationdate><title>Differential expression of amiloride-sensitive NA + channel subunits messenger RNA in the rat uterus</title><author>Pinto, Francisco M. ; Cintado, Cristina G. ; Mérida, Angel ; Hidalgo, Agustin ; Candenas, M.Luz</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-10e6e0a5029b2766f8acd20a28f454aee423a81b1be821a029aeb5f229b95cc43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amiloride - pharmacology</topic><topic>Animals</topic><topic>Diuretics - pharmacology</topic><topic>epithelial Na + channel</topic><topic>Epithelial Sodium Channels</topic><topic>Female</topic><topic>Gene Expression Regulation</topic><topic>mRNA expression</topic><topic>polymerase chain reaction</topic><topic>Pregnancy</topic><topic>rat uterus</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Sodium Channels - genetics</topic><topic>Sodium Channels - metabolism</topic><topic>Uterus - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pinto, Francisco M.</creatorcontrib><creatorcontrib>Cintado, Cristina G.</creatorcontrib><creatorcontrib>Mérida, Angel</creatorcontrib><creatorcontrib>Hidalgo, Agustin</creatorcontrib><creatorcontrib>Candenas, M.Luz</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pinto, Francisco M.</au><au>Cintado, Cristina G.</au><au>Mérida, Angel</au><au>Hidalgo, Agustin</au><au>Candenas, M.Luz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential expression of amiloride-sensitive NA + channel subunits messenger RNA in the rat uterus</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>2000-04-21</date><risdate>2000</risdate><volume>66</volume><issue>22</issue><spage>PL313</spage><epage>PL317</epage><pages>PL313-PL317</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>The expression of the amiloride-sensitive Na + channel was investigated by reverse transcription-polymerase chain reaction (RT-PCR) in uteri from pregnant and non-pregnant rats. Three subunits (α,β,γ) of this epithelial Na + channel have been characterized in the rat. All three subunit mRNAs were present in day 16 pregnant uteri while only α and β subunit mRNAs were detected in uteri from non-pregnant or day 1 postpartum rats. The level of expression of the α subunit was similar in day 16 pregnant and non-pregnant animals while the level of expression of the β subunit was higher in pregnant than in non-pregnant rats. These findings show that amiloride-sensitive Na + channels are expressed in the rat uterus and that mRNA expression levels of the α, β and γ subunits are selectively and differentially regulated during pregnancy in the rat.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>10834307</pmid><doi>10.1016/S0024-3205(00)00550-6</doi></addata></record>
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subjects	Amiloride - pharmacology Animals Diuretics - pharmacology epithelial Na + channel Epithelial Sodium Channels Female Gene Expression Regulation mRNA expression polymerase chain reaction Pregnancy rat uterus Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Sodium Channels - genetics Sodium Channels - metabolism Uterus - metabolism
title	Differential expression of amiloride-sensitive NA + channel subunits messenger RNA in the rat uterus
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