General purpose, field-portable cell-based biosensor platform

There are several groups of researchers developing cell-based biosensors for chemical and biological warfare agents based on electrophysiologic monitoring of cells. In order to transition such sensors from the laboratory to the field, a general-purpose hardware and software platform is required. Thi...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2001-09, Vol.16 (7), p.557-564
Main Authors: Gilchrist, Kristin H., Barker, Valerie N., Fletcher, Lauren E., DeBusschere, B.Derek, Ghanouni, Pejman, Giovangrandi, Laurent, Kovacs, Gregory T.A.
Format: Article
Language:English
Subjects:
Action Potentials
Algorithms
Animals
Biosensing Techniques - instrumentation
Biosensing Techniques - statistics & numerical data
Biosensor
Cell Line
Equipment Design
Extracellular recording
Mice
Myocardium - cytology
Myocardium - metabolism
Portable
Software
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Summary:There are several groups of researchers developing cell-based biosensors for chemical and biological warfare agents based on electrophysiologic monitoring of cells. In order to transition such sensors from the laboratory to the field, a general-purpose hardware and software platform is required. This paper describes the design, implementation, and field-testing of such a system, consisting of cell-transport and data acquisition instruments. The cell-transport module is a self-contained, battery-powered instrument that allows various types of cell-based modules to be maintained at a preset temperature and ambient CO 2 level while in transit or in the field. The data acquisition module provides 32 channels of action potential amplification, filtering, and real-time data streaming to a laptop computer. At present, detailed analysis of the data acquired is carried out off-line, but sufficient computing power is available in the data acquisition module to enable the most useful algorithms to eventually be run real-time in the field. Both modules have sufficient internal power to permit realistic field-testing, such as the example presented in this paper.
ISSN:0956-5663
1873-4235
DOI:10.1016/S0956-5663(01)00169-5