In vitro correlate of immunity in a rabbit model of inhalational anthrax

A serological correlate of vaccine-induced immunity was identified in the rabbit model of inhalational anthrax. Animals were inoculated intramuscularly at 0 and 4 weeks with varying doses of Anthrax Vaccine Adsorbed (AVA) ranging from a human dose to a 1:256 dilution in phosphate-buffered saline (PB...

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Bibliographic Details
Published in:Vaccine 2001-09, Vol.19 (32), p.4768-4773
Main Authors: Pitt, M.L.M, Little, S.F, Ivins, B.E, Fellows, P, Barth, J, Hewetson, J, Gibbs, P, Dertzbaugh, M, Friedlander, A.M
Format: Article
Language:English
Subjects:
Administration, Inhalation
Aerosols
Animals
Anthrax
Anthrax - immunology
Anthrax - prevention & control
Anthrax - transmission
Anthrax Vaccines - immunology
Antibodies, Bacterial - biosynthesis
Antibodies, Bacterial - immunology
Antigens, Bacterial - immunology
Bacillus anthracis
Bacillus anthracis - immunology
Bacterial Capsules - immunology
Bacterial diseases
Bacterial Toxins - immunology
Biological and medical sciences
Enzyme-Linked Immunosorbent Assay
Experimental bacterial diseases and models
Female
Fundamental and applied biological sciences. Psychology
Fundamental immunology
General aspects. Ontogeny. Phylogeny
Immunobiology
Immunoglobulin G - biosynthesis
Immunoglobulin G - immunology
Infectious diseases
Injections, Intramuscular
Male
Mammals
Medical sciences
Models, Animal
Neutralization Tests
Rabbits
Serological correlate
Species Specificity
Specific Pathogen-Free Organisms
Spores, Bacterial
Virulence
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Summary:A serological correlate of vaccine-induced immunity was identified in the rabbit model of inhalational anthrax. Animals were inoculated intramuscularly at 0 and 4 weeks with varying doses of Anthrax Vaccine Adsorbed (AVA) ranging from a human dose to a 1:256 dilution in phosphate-buffered saline (PBS). At 6 and 10 weeks, both the quantitative anti-protective antigen (PA) IgG ELISA and the toxin-neutralizing antibody (TNA) assays were used to measure antibody levels to PA. Rabbits were aerosol-challenged at 10 weeks with a lethal dose (84–133 LD 50) of Bacillus anthracis spores. All the rabbits that received the undiluted and 1:4 dilution of vaccine survived, whereas those receiving the higher dilutions of vaccine (1:16, 1:64 and 1:256) had deaths in their groups. Results showed that antibody levels to PA at both 6 and 10 weeks were significant ( P
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(01)00234-1