Transcriptional reprogramming of dendritic cells by differentiation stimuli

Immature and mature dendritic cells (DC) have been well characterized functionally and phenotypically. Microorganisms or bacterial products such as lipopolysaccharide (LPS) and inflammatory molecules, including tumor necrosis factor (TNF‐α), are both believed to activate the DC maturation program wh...

Full description

Saved in:
Bibliographic Details
Published in:European journal of immunology 2001-09, Vol.31 (9), p.2539-2546
Main Authors: Granucci, Francesca, Vizzardelli, Caterina, Virzi, Ettore, Rescigno, Maria, Ricciardi‐Castagnoli, Paola
Format: Article
Language:English
Subjects:
Animals
Antigen Presentation
Apoptosis
Cell Cycle
Cell Differentiation
Cell Line
Cytokines - biosynthesis
Dendritic cell
Dendritic Cells - cytology
Dendritic Cells - drug effects
Dendritic Cells - immunology
Gene Expression Profiling
Histocompatibility Antigens - immunology
Inflammation - genetics
Inflammation - immunology
Inflammatory stimulus
lipopolysaccharides
Lipopolysaccharides - pharmacology
Mice
Microarray
Oligonucleotide Array Sequence Analysis
oligonucleotides
Peptides - immunology
RNA, Messenger - biosynthesis
Transcription analysis
Transcriptional Activation
Tumor Necrosis Factor-alpha - pharmacology
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Immature and mature dendritic cells (DC) have been well characterized functionally and phenotypically. Microorganisms or bacterial products such as lipopolysaccharide (LPS) and inflammatory molecules, including tumor necrosis factor (TNF‐α), are both believed to activate the DC maturation program which allows DC to initiate and amplify innate and adaptive immune responses. However, there is increasing evidence that the functional state of DC, induced by different stimuli, may be relevant for the immune response outcome. Thus, we compared the transcriptional program of mature, transitional and immature DC, after either LPS or TNF‐α stimulation. GeneChip® oligonucleotide microarrays, representing approximately 6,500 murine genes and ESTs, were used for this analysis. A very diverse modulation of gene expression was observed with the two stimuli. Only LPS‐treated cells showed a pattern of expression of genes compatible with a definitive growth arrest and with a suitable activation and control of the immune response.
ISSN:0014-2980
1521-4141
DOI:10.1002/1521-4141(200109)31:9<2539::AID-IMMU2539>3.0.CO;2-9