Loading…
Mitochondrial ATP synthase 6 as an endogenous control in the quantitative RT-PCR analysis of clinical cancer samples
Real-time polymerase chain reaction (PCR) is a powerful new technique in the evolution of quantitative reverse transcription-PCR assays. With the increased sensitivity and resolution of real-time techniques, the requirements for constitutive expression of endogenous controls have become increasingly...
Saved in:
Published in: | Molecular diagnosis 2000-03, Vol.5 (1), p.39-46 |
---|---|
Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c1308-a9aa63a7ecf2172cea6cbae338d2e96a3ce121bbea834630b39129f873b2707b3 |
---|---|
cites | cdi_FETCH-LOGICAL-c1308-a9aa63a7ecf2172cea6cbae338d2e96a3ce121bbea834630b39129f873b2707b3 |
container_end_page | 46 |
container_issue | 1 |
container_start_page | 39 |
container_title | Molecular diagnosis |
container_volume | 5 |
creator | Gerard, C J Andrejka, L M Macina, R A |
description | Real-time polymerase chain reaction (PCR) is a powerful new technique in the evolution of quantitative reverse transcription-PCR assays. With the increased sensitivity and resolution of real-time techniques, the requirements for constitutive expression of endogenous controls have become increasingly stringent.
We compare the expression of the mitochondrial gene, adenosine triphosphate synthase 6 (ATPsy6), to the expression of other routinely used endogenous control genes (e.g., beta-actin, glyceraldehyde-3-phosphate dehydrogenase [GAPDH], ribosomal RNA 18S [18S rRNA], and cyclophilin). In a diverse assortment of tissues and across a wide range of disease stages, ATPsy6 shows a relative steady state of expression compared with other endogenous controls. ATPsy6 gene expression has been used as an endogenous control in a quantitative real-time PCR assay designed to evaluate the expression of potential cancer diagnostic leads across a diverse tissue panel.
Mitochondrial ATPsy6 serves as a good endogenous control to measure target gene expression independent of the tissue- or disease-specific variation inherent with many housekeeping genes. |
doi_str_mv | 10.1007/BF03262021 |
format | article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71150857</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>71150857</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1308-a9aa63a7ecf2172cea6cbae338d2e96a3ce121bbea834630b39129f873b2707b3</originalsourceid><addsrcrecordid>eNpNkE1LAzEQQHNQbK1e_AGSkwdhNR_tJnusxapQsZR6XmbTWRvZTdokK_Tfu1JBT3OYNw_mEXLF2R1nTN0_zJkUuWCCn5AhZ3qc6UkhBuQ8xk_GOJ8U8owM-oVUTOshSa82ebP1bhMsNHS6XtJ4cGkLEWlOIVJwFN3Gf6DzXaTGuxR8Q62jaYt034FLNkGyX0hX62w5W_UH0ByijdTX1DTWWdN7DTiDgUZodw3GC3JaQxPx8neOyPv8cT17zhZvTy-z6SIzXDKdQQGQS1BoasGVMAi5qQCl1BuBRQ7SIBe8qhC0HOeSVbLgoqi1kpVQTFVyRG6O3l3w-w5jKlsbDTYNOOy_KVXfg-mJ6sHbI2iCjzFgXe6CbSEcSs7Kn67lX9cevv61dlWLm3_oMar8Bi_edTQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71150857</pqid></control><display><type>article</type><title>Mitochondrial ATP synthase 6 as an endogenous control in the quantitative RT-PCR analysis of clinical cancer samples</title><source>Springer Nature</source><creator>Gerard, C J ; Andrejka, L M ; Macina, R A</creator><creatorcontrib>Gerard, C J ; Andrejka, L M ; Macina, R A</creatorcontrib><description>Real-time polymerase chain reaction (PCR) is a powerful new technique in the evolution of quantitative reverse transcription-PCR assays. With the increased sensitivity and resolution of real-time techniques, the requirements for constitutive expression of endogenous controls have become increasingly stringent.
We compare the expression of the mitochondrial gene, adenosine triphosphate synthase 6 (ATPsy6), to the expression of other routinely used endogenous control genes (e.g., beta-actin, glyceraldehyde-3-phosphate dehydrogenase [GAPDH], ribosomal RNA 18S [18S rRNA], and cyclophilin). In a diverse assortment of tissues and across a wide range of disease stages, ATPsy6 shows a relative steady state of expression compared with other endogenous controls. ATPsy6 gene expression has been used as an endogenous control in a quantitative real-time PCR assay designed to evaluate the expression of potential cancer diagnostic leads across a diverse tissue panel.
Mitochondrial ATPsy6 serves as a good endogenous control to measure target gene expression independent of the tissue- or disease-specific variation inherent with many housekeeping genes.</description><identifier>ISSN: 1084-8592</identifier><identifier>DOI: 10.1007/BF03262021</identifier><identifier>PMID: 10837088</identifier><language>eng</language><publisher>United States</publisher><subject>Deoxyribonucleases - metabolism ; Gene Expression Profiling ; Humans ; Mitochondria - enzymology ; Mitochondria - genetics ; Mitochondrial Proton-Translocating ATPases ; Neoplasms - genetics ; Neoplasms - metabolism ; Proton-Translocating ATPases - analysis ; Proton-Translocating ATPases - genetics ; Quality Control ; Reference Standards ; Reverse Transcriptase Polymerase Chain Reaction - methods ; Tissue Distribution</subject><ispartof>Molecular diagnosis, 2000-03, Vol.5 (1), p.39-46</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1308-a9aa63a7ecf2172cea6cbae338d2e96a3ce121bbea834630b39129f873b2707b3</citedby><cites>FETCH-LOGICAL-c1308-a9aa63a7ecf2172cea6cbae338d2e96a3ce121bbea834630b39129f873b2707b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10837088$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gerard, C J</creatorcontrib><creatorcontrib>Andrejka, L M</creatorcontrib><creatorcontrib>Macina, R A</creatorcontrib><title>Mitochondrial ATP synthase 6 as an endogenous control in the quantitative RT-PCR analysis of clinical cancer samples</title><title>Molecular diagnosis</title><addtitle>Mol Diagn</addtitle><description>Real-time polymerase chain reaction (PCR) is a powerful new technique in the evolution of quantitative reverse transcription-PCR assays. With the increased sensitivity and resolution of real-time techniques, the requirements for constitutive expression of endogenous controls have become increasingly stringent.
We compare the expression of the mitochondrial gene, adenosine triphosphate synthase 6 (ATPsy6), to the expression of other routinely used endogenous control genes (e.g., beta-actin, glyceraldehyde-3-phosphate dehydrogenase [GAPDH], ribosomal RNA 18S [18S rRNA], and cyclophilin). In a diverse assortment of tissues and across a wide range of disease stages, ATPsy6 shows a relative steady state of expression compared with other endogenous controls. ATPsy6 gene expression has been used as an endogenous control in a quantitative real-time PCR assay designed to evaluate the expression of potential cancer diagnostic leads across a diverse tissue panel.
Mitochondrial ATPsy6 serves as a good endogenous control to measure target gene expression independent of the tissue- or disease-specific variation inherent with many housekeeping genes.</description><subject>Deoxyribonucleases - metabolism</subject><subject>Gene Expression Profiling</subject><subject>Humans</subject><subject>Mitochondria - enzymology</subject><subject>Mitochondria - genetics</subject><subject>Mitochondrial Proton-Translocating ATPases</subject><subject>Neoplasms - genetics</subject><subject>Neoplasms - metabolism</subject><subject>Proton-Translocating ATPases - analysis</subject><subject>Proton-Translocating ATPases - genetics</subject><subject>Quality Control</subject><subject>Reference Standards</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>Tissue Distribution</subject><issn>1084-8592</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNpNkE1LAzEQQHNQbK1e_AGSkwdhNR_tJnusxapQsZR6XmbTWRvZTdokK_Tfu1JBT3OYNw_mEXLF2R1nTN0_zJkUuWCCn5AhZ3qc6UkhBuQ8xk_GOJ8U8owM-oVUTOshSa82ebP1bhMsNHS6XtJ4cGkLEWlOIVJwFN3Gf6DzXaTGuxR8Q62jaYt034FLNkGyX0hX62w5W_UH0ByijdTX1DTWWdN7DTiDgUZodw3GC3JaQxPx8neOyPv8cT17zhZvTy-z6SIzXDKdQQGQS1BoasGVMAi5qQCl1BuBRQ7SIBe8qhC0HOeSVbLgoqi1kpVQTFVyRG6O3l3w-w5jKlsbDTYNOOy_KVXfg-mJ6sHbI2iCjzFgXe6CbSEcSs7Kn67lX9cevv61dlWLm3_oMar8Bi_edTQ</recordid><startdate>200003</startdate><enddate>200003</enddate><creator>Gerard, C J</creator><creator>Andrejka, L M</creator><creator>Macina, R A</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200003</creationdate><title>Mitochondrial ATP synthase 6 as an endogenous control in the quantitative RT-PCR analysis of clinical cancer samples</title><author>Gerard, C J ; Andrejka, L M ; Macina, R A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1308-a9aa63a7ecf2172cea6cbae338d2e96a3ce121bbea834630b39129f873b2707b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Deoxyribonucleases - metabolism</topic><topic>Gene Expression Profiling</topic><topic>Humans</topic><topic>Mitochondria - enzymology</topic><topic>Mitochondria - genetics</topic><topic>Mitochondrial Proton-Translocating ATPases</topic><topic>Neoplasms - genetics</topic><topic>Neoplasms - metabolism</topic><topic>Proton-Translocating ATPases - analysis</topic><topic>Proton-Translocating ATPases - genetics</topic><topic>Quality Control</topic><topic>Reference Standards</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - methods</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gerard, C J</creatorcontrib><creatorcontrib>Andrejka, L M</creatorcontrib><creatorcontrib>Macina, R A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular diagnosis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gerard, C J</au><au>Andrejka, L M</au><au>Macina, R A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mitochondrial ATP synthase 6 as an endogenous control in the quantitative RT-PCR analysis of clinical cancer samples</atitle><jtitle>Molecular diagnosis</jtitle><addtitle>Mol Diagn</addtitle><date>2000-03</date><risdate>2000</risdate><volume>5</volume><issue>1</issue><spage>39</spage><epage>46</epage><pages>39-46</pages><issn>1084-8592</issn><abstract>Real-time polymerase chain reaction (PCR) is a powerful new technique in the evolution of quantitative reverse transcription-PCR assays. With the increased sensitivity and resolution of real-time techniques, the requirements for constitutive expression of endogenous controls have become increasingly stringent.
We compare the expression of the mitochondrial gene, adenosine triphosphate synthase 6 (ATPsy6), to the expression of other routinely used endogenous control genes (e.g., beta-actin, glyceraldehyde-3-phosphate dehydrogenase [GAPDH], ribosomal RNA 18S [18S rRNA], and cyclophilin). In a diverse assortment of tissues and across a wide range of disease stages, ATPsy6 shows a relative steady state of expression compared with other endogenous controls. ATPsy6 gene expression has been used as an endogenous control in a quantitative real-time PCR assay designed to evaluate the expression of potential cancer diagnostic leads across a diverse tissue panel.
Mitochondrial ATPsy6 serves as a good endogenous control to measure target gene expression independent of the tissue- or disease-specific variation inherent with many housekeeping genes.</abstract><cop>United States</cop><pmid>10837088</pmid><doi>10.1007/BF03262021</doi><tpages>8</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1084-8592 |
ispartof | Molecular diagnosis, 2000-03, Vol.5 (1), p.39-46 |
issn | 1084-8592 |
language | eng |
recordid | cdi_proquest_miscellaneous_71150857 |
source | Springer Nature |
subjects | Deoxyribonucleases - metabolism Gene Expression Profiling Humans Mitochondria - enzymology Mitochondria - genetics Mitochondrial Proton-Translocating ATPases Neoplasms - genetics Neoplasms - metabolism Proton-Translocating ATPases - analysis Proton-Translocating ATPases - genetics Quality Control Reference Standards Reverse Transcriptase Polymerase Chain Reaction - methods Tissue Distribution |
title | Mitochondrial ATP synthase 6 as an endogenous control in the quantitative RT-PCR analysis of clinical cancer samples |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T13%3A47%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Mitochondrial%20ATP%20synthase%206%20as%20an%20endogenous%20control%20in%20the%20quantitative%20RT-PCR%20analysis%20of%20clinical%20cancer%20samples&rft.jtitle=Molecular%20diagnosis&rft.au=Gerard,%20C%20J&rft.date=2000-03&rft.volume=5&rft.issue=1&rft.spage=39&rft.epage=46&rft.pages=39-46&rft.issn=1084-8592&rft_id=info:doi/10.1007/BF03262021&rft_dat=%3Cproquest_cross%3E71150857%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c1308-a9aa63a7ecf2172cea6cbae338d2e96a3ce121bbea834630b39129f873b2707b3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=71150857&rft_id=info:pmid/10837088&rfr_iscdi=true |