The titanium surface texture effects adherence and growth of human gingival keratinocytes and human maxillar osteoblast-like cells in vitro

• Published in: Biomaterials 2001-10, Vol.22 (20), p.2799-2809
• Main Authors: Lauer, G, Wiedmann-Al-Ahmad, M, Otten, J.E, Hübner, U, Schmelzeisen, R, Schilli, W
• Format: Article
• Language: English
• Subjects: Adhesion; Alkaline Phosphatase - metabolism; Biological and medical sciences; Cell Adhesion; Cell Division; Cells, Cultured; Collagen - metabolism; Culture Media; Dental Implants; Enzyme-Linked Immunosorbent Assay; Gingiva - cytology; Gingiva - metabolism; Gingiva - ultrastructure; Human gingival keratinocytes; Human maxillar osteoblast-like cells; Humans; Immunohistochemistry; In Vitro Techniques; Keratinocytes - cytology; Keratinocytes - metabolism; Keratinocytes - ultrastructure; Maxilla - cytology; Medical sciences; Microscopy, Electron, Scanning; Osteoblasts - cytology; Osteoblasts - enzymology; Osteoblasts - metabolism; Osteoblasts - ultrastructure; Primary and secondary culture; Proliferation; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects); Surface Properties; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; Technology. Biomaterials. Equipments. Material. Instrumentation; Titanium; Titanium surface textures
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/S0142-9612(01)00024-2

The adhesion, orientation and proliferation of human gingival epithelial cells and human maxillar osteoblast-like cells in primary and secondary culture were studied on glossy polished, sandblasted and plasma-sprayed titanium surfaces by scanning electron microscopy and in thin sections. The primary cultured explants of human gingival epithelial cells attached, spread and proliferated on all titanium surfaces with the greatest extension on the polished and the smallest extension on plasma-sprayed surfaces. In secondary suspension cultures of gingival keratinocytes, attachment spreading and growth was only observed on polished and plasma-sprayed surfaces, but not on sandblasted surfaces. Moreover, the attachment of these cells depended on the seeding concentration as well as on the coating with fetal calf serum. Cells on polished surfaces developed an extremely flat cell shape, but on sandblasted and plasma-sprayed surfaces a more cuboidal shape. In contrast human maxillar osteoblasts seeded as secondary suspension cultures attached very well to all three differently textured titanium surfaces and showed identical growth patterns independent of the titanium surface structure. These findings suggest that cell morphology, orientation, proliferation and adhesion of human gingival epithelial cells in primary or secondary culture are dependent on the texture of the titanium surface whereas no such differences were observed for maxillar osteoblast-like cells. In conclusion, the soft tissue integration and response is more influenced by the surface texture than the process of osseointegration.