Induction of Potent Th1-Type Immune Responses from a Novel DNA Vaccine for West Nile Virus New York Isolate (WNV-NY1999)

West Nile virus (WNV) is a vectorborne pathogen that induces brain inflammation and death. Recently, confirmed cases of infection and deaths have occurred in the United States Mid-Atlantic region. In this study, a DNA vaccine encoding the WNV capsid protein was constructed, and the in vivo immune re...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of infectious diseases 2001-10, Vol.184 (7), p.809-816
Main Authors: Yang, Joo-Sung, Kim, J. Joseph, Hwang, Daniel, Choo, Andrew Y., Dang, Kesen, Maguire, Henry, Kudchodkar, Sagar, Ramanathan, Mathura P., Weiner, David B.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antigens, Viral - immunology
Biological and medical sciences
Capsid - immunology
CD4 antigen
CD8 antigen
Chemokines
Cytokines
Cytokines - analysis
Disease Models, Animal
DNA
DNA vaccines
Female
Fundamental and applied biological sciences. Psychology
Human viral diseases
Immunization
Infectious diseases
macrophage inflammatory protein 1^b
Major Articles
Medical sciences
Mice
Mice, Inbred BALB C
Microbiology
Muscles - immunology
Plasmids
T lymphocytes
T-Lymphocytes, Cytotoxic - immunology
Th1 Cells - immunology
Vaccination
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, DNA - administration & dosage
Viral diseases
Viral diseases of the lymphoid tissue and the blood. Aids
Viral Vaccines - administration & dosage
Virology
West Nile Fever - prevention & control
West Nile virus
West Nile virus - immunology
West Nile virus - isolation & purification
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:West Nile virus (WNV) is a vectorborne pathogen that induces brain inflammation and death. Recently, confirmed cases of infection and deaths have occurred in the United States Mid-Atlantic region. In this study, a DNA vaccine encoding the WNV capsid protein was constructed, and the in vivo immune responses generated were investigated in DNA vaccine–immunized mice. Antigen-specific humoral and cellular immune responses were observed, including a potent induction of antigen-specific Th1 and cytotoxic T lymphocyte responses. Strong induction of Th1-type immune responses included high levels of antigen-specific elaboration of the Th1-type cytokines interferon-γ and interleukin-2 and β-chemokines RANTES (regulated upon activation, normal T cell-expressed and secreted) and macrophage inflammatory protein–1β. Dramatic infiltration of CD4 and CD8 T cells and macrophages also was observed at the muscle injection site. These results support the potential utility of this method as a tool for developing immunization strategies for WNV and other emerging pathogens
ISSN:0022-1899
1537-6613
DOI:10.1086/323395