A multiplex polymerase chain reaction to differentiate β-lactamase plasmids of Neisseria gonorrhoeae

In penicillinase-producing Neisseria gonorrhoeae (PPNG), resistance to penicillin may be mediated by one of several related plasmids of different sizes. These include the Asian, African and Rio/Toronto plasmids. Identification of these plasmids provides useful epidemiological information, but has ne...

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Bibliographic Details
Published in:Journal of antimicrobial chemotherapy 2000-06, Vol.45 (6), p.777-782
Main Authors: Palmer, H. M., Leeming, J. P., Turner, A.
Format: Article
Language:English
Subjects:
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
beta-Lactamases - genetics
Biological and medical sciences
Biotechnology
DNA Primers
DNA, Bacterial - genetics
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
In vitro gene amplification. Pcr technique
Medical sciences
Methods. Procedures. Technologies
Neisseria gonorrhoeae
Neisseria gonorrhoeae - enzymology
Neisseria gonorrhoeae - genetics
Penicillinase - biosynthesis
Penicillinase - genetics
Pharmacology. Drug treatments
Plasmids - genetics
Polymerase Chain Reaction
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Summary:In penicillinase-producing Neisseria gonorrhoeae (PPNG), resistance to penicillin may be mediated by one of several related plasmids of different sizes. These include the Asian, African and Rio/Toronto plasmids. Identification of these plasmids provides useful epidemiological information, but has necessitated plasmid purification and gel analysis. We have developed a rapid, simple multiplex polymerase chain reaction (PCR) which discriminates between the β-lactamase resistance plasmids that are frequently found in strains of N. gonorrhoeae. Amplicons of 1191, 958 and 650 bp were produced from strains containing the African, Asian and Rio/Toronto plasmids, respectively, whilst no products resulted from non-PPNG strains harbouring the cryptic, conjugative or tetracycline resistance plasmids. PCR analysis of 123 strains of PPNG identified 60 strains with African, 16 strains with Asian and 47 strains with Rio/Toronto plasmids and showed complete agreement with the standard plasmid analysis.
ISSN:0305-7453
1460-2091
DOI:10.1093/jac/45.6.777