Detection of Progesterone Receptor Transcript in Human Spermatozoa

The present study, to our knowledge, is the first to demonstrate presence of progesterone receptor (PR) transcript in human spermatozoa. The study shows the presence of low copy number PR mRNA in mature human spermatozoa. The PR transcript in spermatozoa was detected by reverse transcriptase-polymer...

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Bibliographic Details
Published in:Biology of reproduction 2000-06, Vol.62 (6), p.1610-1614
Main Authors: Sachdeva, Geetanjali, Shah, Chirag Ashwinkumar, Kholkute, Sanjiva Dattatraya, Puri, Chander Prakash
Format: Article
Language:English
Subjects:
Base Sequence
Binding Sites
Biological and medical sciences
Contents
DNA - chemistry
DNA - metabolism
Fundamental and applied biological sciences. Psychology
Hormone metabolism and regulation
Humans
Male
Mammalian male genital system
Molecular Sequence Data
Progesterone - metabolism
Receptors, Progesterone - genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
Spermatozoa - chemistry
Vertebrates: reproduction
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Summary:The present study, to our knowledge, is the first to demonstrate presence of progesterone receptor (PR) transcript in human spermatozoa. The study shows the presence of low copy number PR mRNA in mature human spermatozoa. The PR transcript in spermatozoa was detected by reverse transcriptase-polymerase chain reaction using primers specific for the hormone binding domain and the DNA binding domain of the conventional uterine PR. Further, the cDNA sequence of the partial PR transcript from spermatozoa was found to be identical to the region spanning nucleotides 2694 to 3230 of the conventional PR full-length cDNA sequence. This study also indirectly suggests that the PR protein indeed is an intrinsic sperm protein and is not acquired through proteinaceous secretions of accessory reproductive organs.
ISSN:0006-3363
1529-7268
DOI:10.1095/biolreprod62.6.1610