The Xenopus eomesodermin promoter and its concentration-dependent response to activin

Eomesodermin is an essential early gene in Xenopus mesoderm formation and shows a morphogen-like response to activin. Here we define the regions of the Eomesodermin promoter required for mesodermal expression and for concentration-dependent response to activin. We find an activin response element (A...

Full description

Saved in:
Bibliographic Details
Published in:Mechanisms of development 2000-06, Vol.94 (1-2), p.133-146
Main Authors: Ryan, K, Garrett, N, Bourillot, P, Stennard, F, Gurdon, J B
Format: Article
Language:English
Subjects:
Activins
Animals
Base Sequence
Binding Sites
DNA-Binding Proteins - metabolism
Dose-Response Relationship, Drug
Embryo, Nonmammalian
Endoderm - physiology
Forkhead Transcription Factors
Genes, Reporter
Globins - genetics
Globins - metabolism
Inhibins - metabolism
Inhibins - pharmacology
Mesoderm - physiology
Molecular Sequence Data
Promoter Regions, Genetic
Repressor Proteins - genetics
Repressor Proteins - metabolism
Response Elements - drug effects
Response Elements - genetics
Signal Transduction
T-Box Domain Proteins - drug effects
T-Box Domain Proteins - genetics
T-Box Domain Proteins - metabolism
Transcription Factors - metabolism
Xenopus - embryology
Xenopus - genetics
Xenopus Proteins
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Eomesodermin is an essential early gene in Xenopus mesoderm formation and shows a morphogen-like response to activin. Here we define the regions of the Eomesodermin promoter required for mesodermal expression and for concentration-dependent response to activin. We find an activin response element (ARE) located between -5.6 and -5.0 kb which contains two critical FAST2 binding sites. The ARE alone is necessary and sufficient for concentration-dependent response to activin. A 5.6 kb promoter recapitulates Eomes expression in normal mesoderm cells. A repressor element extinguishes Eomes expression in the endoderm. We relate our results to mesoderm patterning in early Xenopus development and to a mechanism of morphogen gradient response.
ISSN:0925-4773
DOI:10.1016/S0925-4773(00)00300-2