Characterization of C18-bonded liquid chromatographic stationary phases by Raman spectroscopy: the effect of mobile phase composition

Raman spectroscopy is used to examine the effect of mobile phase composition on the orientation of octadecyl-bonded silica-based reversed-phase liquid chromatographic (RPLC) stationary phase ligands. The effect of ligand bonding density is also investigated. The present experimental set-up utilizes...

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Bibliographic Details
Published in:Journal of chromatography. A 2000-04, Vol.877 (1-2), p.25-39
Main Authors: Doyle, C A, Vickers, T J, Mann, C K, Dorsey, J G
Format: Article
Language:English
Subjects:
Chloroform - chemistry
Chromatography, Liquid - instrumentation
Ligands
Methanol - chemistry
Molecular Conformation
Solvents - chemistry
Spectrum Analysis, Raman
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Summary:Raman spectroscopy is used to examine the effect of mobile phase composition on the orientation of octadecyl-bonded silica-based reversed-phase liquid chromatographic (RPLC) stationary phase ligands. The effect of ligand bonding density is also investigated. The present experimental set-up utilizes a direct, noninvasive, on-column approach to examine the solvent dependent conformational behavior of the bonded ligands under flow-rate and back pressure conditions similar to those used during conventional RPLC measurements. Neat, single-component, mobile phase solvents including water, acetonitrile, methanol and chloroform are used to investigate the hypothesized collapsing and extension of stationary phase ligands with changes in mobile phase composition. No evidence of phase collapse was observed upon changing the mobile phase composition from an organic to an aqueous content. Also, Raman spectroscopic measurements allowed the differentiation between associated and free acetonitrile solvent.
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)00175-8