Threshold levels of gene expression of the melanoma antigen gp100 correlate with tumor cell recognition by cytotoxic T lymphocytes

The level of expression of melanoma antigens (MA) may modulate the host immunologic response. Thus, the accurate measurement of MA expression may allow proper patient selection for antigen‐specific therapies and yield important information for the evaluation of clinical results. In this study, we me...

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Bibliographic Details
Published in:International journal of cancer 2000-06, Vol.86 (6), p.818-826
Main Authors: Riker, Adam I., Kammula, Udai S., Panelli, Monica C., Wang, Ena, Ohnmacht, Galen A., Steinberg, Seth M., Rosenberg, Steven A., Marincola, Francesco M.
Format: Article
Language:English
Subjects:
Antigens, Neoplasm - genetics
Base Sequence
Biological and medical sciences
Humans
Medical sciences
Melanoma - immunology
Melanoma-Specific Antigens
Molecular Sequence Data
Multiple tumors. Solid tumors. Tumors in childhood (general aspects)
Neoplasm Proteins - analysis
Neoplasm Proteins - genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
T-Lymphocytes, Cytotoxic - immunology
Tumor Cells, Cultured
Tumors
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Summary:The level of expression of melanoma antigens (MA) may modulate the host immunologic response. Thus, the accurate measurement of MA expression may allow proper patient selection for antigen‐specific therapies and yield important information for the evaluation of clinical results. In this study, we measured the absolute levels of MA messenger ribonucleic acid (mRNA) in tumor cell lines utilizing real‐time quantitative reverse transcriptase‐polymerase chain reaction (qRT‐PCR). mRNA levels of MART‐1, gp100, tyrosinase, TRP‐1 and TRP‐2 melanoma differentiation antigens and MAGE‐1, MAGE‐3 and ESO‐1 cancer testis (CT) antigens were compared in 24 early‐passage (
ISSN:0020-7136
1097-0215
DOI:10.1002/(SICI)1097-0215(20000615)86:6<818::AID-IJC10>3.0.CO;2-W