Complete Coding Sequence of the Alkhurma Virus, a Tick-Borne Flavivirus Causing Severe Hemorrhagic Fever in Humans in Saudi Arabia

To date, tick-borne flaviviruses responsible for hemorrhagic fever in humans have been isolated in Siberia (Omsk hemorrhagic fever virus), India (Kyasanur Forest disease virus, KFDV), and in Saudi Arabia (Alkhurma virus, ALKV). Prior to this study, only partial coding sequences of these severe patho...

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Published in:Biochemical and biophysical research communications 2001-09, Vol.287 (2), p.455-461
Main Authors: Charrel, Rémi N., Zaki, Ali Mohamed, Attoui, Houssam, Fakeeh, Mazen, Billoir, Frédérique, Yousef, Amany Ibrahim, de Chesse, Reine, De Micco, Philippe, Gould, Ernest A., de Lamballerie, Xavier
Format: Article
Language:English
Subjects:
Alkhurma virus
Flaviviridae
Flavivirus
Flavivirus - classification
Flavivirus - genetics
hemorrhagic fever
Hemorrhagic Fevers, Viral - virology
Humans
Open Reading Frames
Phylogeny
Saudi Arabia
Tick-Borne Diseases - virology
tick-borne virus
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Summary:To date, tick-borne flaviviruses responsible for hemorrhagic fever in humans have been isolated in Siberia (Omsk hemorrhagic fever virus), India (Kyasanur Forest disease virus, KFDV), and in Saudi Arabia (Alkhurma virus, ALKV). Prior to this study, only partial coding sequences of these severe pathogens had been determined. We report here the complete coding sequence of ALK virus, which was determined to be 10,248 nucleotides (nt) long, and to encode a single 3,416 amino acid polyprotein. Independent analyses of the complete polyprotein and the envelope protein provided genetic and phylogenetic evidence that ALKV belongs to the tick-borne flavivirus group, within which it is most closely related to KFDV. Analysis of structural genes, genetic distances, and evolutionary relationship indicate that ALKV and KFDV derived from a common phylogenetic ancestor and constitute two genetic subtypes of the same virus species according to current genetic criteria of classification.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2001.5610