Effect of serum from different patient populations on the serum bactericidal test

We evaluated the effect of serum from normal and uraemic volunteers, neutropenic patients and burn patients on the serum bactericidal test. Serum samples were spiked with ceftazidime to mimic in vivo peak (75 mg/L) and trough (5 mg/L) concentrations. Serum inhibitory and bactericidal titres (SIT and...

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Bibliographic Details
Published in:Journal of antimicrobial chemotherapy 2001-09, Vol.48 (3), p.417-420
Main Authors: Kays, Michael B., White, Roger L., Friedrich, Lawrence V.
Format: Article
Language:English
Subjects:
Adult
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
Biological and medical sciences
Burns - blood
Ceftazidime - blood
Ceftazidime - pharmacology
Cephalosporins - blood
Cephalosporins - pharmacology
Escherichia coli
Escherichia coli - drug effects
Female
Humans
Male
Medical sciences
Microbial Sensitivity Tests
Middle Aged
Neutropenia - blood
Pharmacology. Drug treatments
Pseudomonas aeruginosa
Pseudomonas aeruginosa - drug effects
Serum Bactericidal Test
Uremia - blood
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Summary:We evaluated the effect of serum from normal and uraemic volunteers, neutropenic patients and burn patients on the serum bactericidal test. Serum samples were spiked with ceftazidime to mimic in vivo peak (75 mg/L) and trough (5 mg/L) concentrations. Serum inhibitory and bactericidal titres (SIT and SBT) were performed in triplicate using Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853. For E. coli, the trough SIT and SBT were significantly higher in serum from burn patients compared with normal volunteers (P ≤ 0.024). The trough SIT was significantly higher in serum from burn patients compared with neutropenic patients (P = 0.022) and in uraemic patients compared with normal volunteers (P = 0.04). No significant differences between subject populations were found for P. aeruginosa.
ISSN:0305-7453
1460-2091
1460-2091
DOI:10.1093/jac/48.3.417