Detection of active hepatitis C virus and hepatitis G virus/gb virus C replication in bone marrow in human subjects

We have analyzed the presence of hepatitis C virus (HCV) and hepatitis G virus (HGV) sequences in bone marrow and serum samples from 48 patients of a hematologic outpatient clinic. HCV RNA was detected in 18 (38%) and 15 (31%) and HGV RNA was detected in 6 (13%) and 9 (19%) of serum and bone marrow...

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Bibliographic Details
Published in:Blood 2000-06, Vol.95 (12), p.3986-3989
Main Authors: RADKOWSKI, M, KUBICKA, J, KISIEL, E, CIANCIARA, J, NOWICKI, M, RAKELA, J, LASKUS, T
Format: Article
Language:English
Subjects:
Adult
Aged
Anemia - pathology
Anemia - virology
Biological and medical sciences
Bone Marrow - pathology
Bone Marrow - virology
Female
Flaviviridae - isolation & purification
Flaviviridae - physiology
Hepacivirus - isolation & purification
Hepacivirus - physiology
Hepatitis B, Chronic - pathology
Hepatitis B, Chronic - virology
Hepatitis C, Chronic - blood
Hepatitis C, Chronic - pathology
Hepatitis C, Chronic - virology
Human viral diseases
Humans
Infectious diseases
Leukemia - pathology
Leukemia - virology
Leukopenia - pathology
Leukopenia - virology
Male
Medical sciences
Middle Aged
Multiple Myeloma - pathology
Multiple Myeloma - virology
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral - blood
RNA, Viral - isolation & purification
Viral diseases
Viral hepatitis
Virus Replication
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Summary:We have analyzed the presence of hepatitis C virus (HCV) and hepatitis G virus (HGV) sequences in bone marrow and serum samples from 48 patients of a hematologic outpatient clinic. HCV RNA was detected in 18 (38%) and 15 (31%) and HGV RNA was detected in 6 (13%) and 9 (19%) of serum and bone marrow samples, respectively. In 3 patients, HGV RNA was detectable in bone marrow but not in the serum; 2 of these patients were negative for the presence of specific antibodies. Using a highly strand-specific Tth-based reverse transcriptase-polymerase chain reaction (RT-PCR), the presence of HCV RNA and HGV RNA negative strand was demonstrated in 4 and 5 bone marrow samples, respectively. Our study shows that HCV and HGV can replicate in bone marrow; in the case of HGV, analysis of serum may underestimate the true prevalence of infection. (Blood. 2000;95:3986-3989)
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.v95.12.3986