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Determination of S-nitrosoglutathione in erythrocytes by capillary zone electrophoresis

A method for separation and quantification of S‐nitrosoglutathione in red cell extracts by capillary electrophoresis is reported. The method is based on the direct analysis of the metaphosphoric acid erythrocyte extract containing diethylenetriaminepentaacetic acid. Optimization of the method is bri...

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Published in:	Electrophoresis 2000-05, Vol.21 (8), p.1606-1610
Main Authors:	Messana, Irene, Rossetti, Diana Valeria, Misiti, Francesco, Vincenzoni, Federica, Tellone, Ester, Giardina, Bruno, Castagnola, Massimo
Format:	Article
Language:	English
Subjects:	Capillary zone electrophoresis Electrophoresis, Capillary - methods Erythrocytes - chemistry Glutathione - analogs & derivatives Glutathione - blood Glutathione - chemistry Glutathione Disulfide - chemistry Humans Nitroso Compounds - blood Oxidized glutathione Red blood cell Reduced glutathione S-Nitrosoglutathione
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creator	Messana, Irene Rossetti, Diana Valeria Misiti, Francesco Vincenzoni, Federica Tellone, Ester Giardina, Bruno Castagnola, Massimo
description	A method for separation and quantification of S‐nitrosoglutathione in red cell extracts by capillary electrophoresis is reported. The method is based on the direct analysis of the metaphosphoric acid erythrocyte extract containing diethylenetriaminepentaacetic acid. Optimization of the method is briefly discussed. Best results in the shortest time were obtained at 25°C, using a coated capillary, 7 kV applied voltage and phosphate sodium 40 mmol/L (pH 2.2) as running buffer. Reproducibility, detection limits, and recoveries of S‐nitrosoglutathione analyses were checked. The results evidenced that S‐nitrosoglutathione is formed in erythrocytes treated with S‐nitrosocysteine, a transnitrosating agent. Under our experimental conditions, the contemporaneous detection and quantification of reduced and oxidized glutathione present in cell extract could also be performed.
doi_str_mv	10.1002/(SICI)1522-2683(20000501)21:8<1606::AID-ELPS1606>3.0.CO;2-2
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subjects	Capillary zone electrophoresis Electrophoresis, Capillary - methods Erythrocytes - chemistry Glutathione - analogs & derivatives Glutathione - blood Glutathione - chemistry Glutathione Disulfide - chemistry Humans Nitroso Compounds - blood Oxidized glutathione Red blood cell Reduced glutathione S-Nitrosoglutathione
title	Determination of S-nitrosoglutathione in erythrocytes by capillary zone electrophoresis
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