Expression of the Erythrocyte-Binding Antigen 175 in Sporozoites and in Liver Stages of Plasmodium falciparum

Screening of a Plasmodium falciparum genomic expression library for antigens expressed at the pre-erythrocytic stages resulted in the isolation of a recombinant phage (DG249) whose insert corresponded to regions II and III of a 175-kDa erythrocyte-binding antigen (EBA-175). EBA-175 is a parasite lig...

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Bibliographic Details
Published in:The Journal of infectious diseases 2001-10, Vol.184 (7), p.892-897
Main Authors: Grüner, Anne Charlotte, Brahimi, Karima, Letourneur, Franck, Rénia, Laurent, Eling, Wijnand, Snounou, Georges, Druilhe, Pierre
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies, Protozoan - blood
Antibodies, Protozoan - immunology
Antigens
Antigens, Protozoan - genetics
Antigens, Protozoan - isolation & purification
Biological and medical sciences
Blotting, Western
Carrier Proteins - genetics
Carrier Proteins - isolation & purification
Erythrocyte invasion
erythrocyte-binding antigen 175
Erythrocytes
Fluorescent Antibody Technique, Indirect
Genomic Library
Human protozoal diseases
Humans
Infectious diseases
Liver
Liver - parasitology
Major Articles
Malaria
Malaria, Falciparum - blood
Malaria, Falciparum - parasitology
Medical sciences
Otorhinolaryngology. Stomatology
Pan troglodytes - parasitology
Parasites
Parasitic diseases
Parasitism
Plasmodium falciparum
Plasmodium falciparum - growth & development
Plasmodium falciparum - immunology
Protozoal diseases
Protozoan Proteins - genetics
Protozoan Proteins - isolation & purification
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
RNA, Protozoan - genetics
Schizonts
Sporozoites
Tumors
Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology
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Summary:Screening of a Plasmodium falciparum genomic expression library for antigens expressed at the pre-erythrocytic stages resulted in the isolation of a recombinant phage (DG249) whose insert corresponded to regions II and III of a 175-kDa erythrocyte-binding antigen (EBA-175). EBA-175 is a parasite ligand implicated in red blood cell invasion. Reverse-transcriptase polymerase chain reaction, indirect immunofluorescent antibody test, and Western blot analysis confirmed that EBA-175 is expressed not only in blood-stage parasites but also in infected hepatocytes and on the sporozoite surface. The presence of EBA-175 on pre-erythrocytic parasites enhances the vaccine potential of this antigen by adding another target to the immune responses elicited by immunization
ISSN:0022-1899
1537-6613
DOI:10.1086/323394