CHARACTERIZATION OF JOK-1, A HUMAN GASTRIC EPITHELIAL CELL LINE

• Published in: In vitro cellular & developmental biology. Animal 2000-04, Vol.36 (4), p.228-234
• Main Authors: OKAYAMA, N, FOWLER, M. R, JENNINGS, S. R, PATEL, P, SPECIAN, R, ALEXANDER, B, JACKSON, T. H, OSHIMA, T, SHANNON, K, ALEXANDER, J. S
• Format: Article
• Language: English
• Subjects: Antigens; Antigens, Polyomavirus Transforming - metabolism; beta Catenin; Blotting, Western; cadherin; Cadherins; Cadherins - metabolism; Cell Line, Transformed - cytology; Cell Line, Transformed - metabolism; Cell lines; CELLULAR MODELS; Cultured cells; cytokeratin; Cytoskeletal Proteins - metabolism; Epithelial cells; Epithelial Cells - cytology; Epithelial Cells - metabolism; Fluorescent Antibody Technique; Genetic Markers; Humans; Keratins - metabolism; Kidney - cytology; Membrane Proteins - metabolism; mucin; Mucins; Occludin; Phosphoproteins - metabolism; Simian virus 40; Simian virus 40 - genetics; Solutes; Stomach; Stomach - cytology; Trans-Activators; Transfection; Vimentin; Vimentin - metabolism; Zonula Occludens-1 Protein; Zonula Occludens-2 Protein
• ISSN: 1071-2690; 1543-706X; 1543-706X
• DOI: 10.1290/1071-2690(2000)036<0228:COJAHG>2.0.CO;2

Human gastric epithelial cells were isolated from samples of human gastric lining and immortalized with simian virus 40 (SV40) to generate the stable human gastric epithelial cell line “JOK-1.” These cells express conventional epithelial markers (vimentin, cytokeratin-18, occludin, N- and E-cadherins, β-catenin, ZO-1, ZO-2, mucin, epithelial specific antigen) as well as SV40 large T-antigen. These cells rapidly externalized E-cadherin in response to acidic medium, and exhibited epithelial-like barrier properties that are also regulated by media pH. In contrast, the kidney epithelial cell line “MDCK” also expresses several epithelial markers (vimentin, cytokeratin-18, occludin, N- and E-cadherin, β-catenin, ZO-1, ZO-2, epithelial specific antigen), but does not express mucin, or large T-antigen. However, MDCK rapidly internalize their E-cadherin from the cell surface and increase the solute flux in an acidic medium. These data suggest that the JOK-1 cell line is a potentially useful cell line for developing models of gastric epithelial function, development, and disease.