Immunohistochemical Analysis and Prognostic Value of Cathepsin D Determination in Laryngeal Squamous Cell Carcinoma

Cathepsin D, a protease with the capability of degrading matrix proteins, is implicated in the process of breast and colorectal cancer invasion and metastasis. Biochemical studies in laryngeal cancer have shown a potential prognostic significance of cathepsin D content determination. We studied immu...

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Bibliographic Details
Published in:Journal of Chemical Information and Computer Sciences 2000-05, Vol.40 (3), p.545-549
Main Authors: Seiwerth, Sven, Štambuk, Nikola, Konjevoda, Paško, Mašić, Nikola, Vasilj, Ankica, Bura, Miljenko, Klapan, Ivo, Manojlović, Spomenka, Đanić, Davor
Format: Article
Language:English
Subjects:
Carcinoma, Squamous Cell - enzymology
Carcinoma, Squamous Cell - pathology
Cathepsin D - metabolism
Humans
Immunohistochemistry
Laryngeal Neoplasms - enzymology
Laryngeal Neoplasms - pathology
Prognosis
Proportional Hazards Models
Survival Analysis
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Summary:Cathepsin D, a protease with the capability of degrading matrix proteins, is implicated in the process of breast and colorectal cancer invasion and metastasis. Biochemical studies in laryngeal cancer have shown a potential prognostic significance of cathepsin D content determination. We studied immunohistochemical positivity of cathepsin D in tumor epithelium and stroma of 61 surgical specimens of squamous cell laryngeal cancer. Immunohistochemical reaction was quantitatively assessed using a PC-based image analysis system SFORM-VAMS. The results were correlated to clinical and morphological parameters and survival. Immunohistochemical positivity was noted in neoplastic cells and tumor stroma. Significant prognostic value for cathepsin D was established separately for epithelial tumor component and tumor stroma using log-rank test, the Cox proportional hazards regression model, and C4.5 machine learning system. In all groups, patients above the median cathepsin D staining showed significantly shorter survival time. C4.5 machine learning system extracted cutoff values for the decision tree that defines the probabilities of patients survival and death with high sensitivity (92.8% alive, 73.6% dead), 100% specificity, and 86.9% accuracy. This makes immunohistochemical cathepsin D estimation an independent prognostic parameter in laryngeal carcinomas within a 5-year period from the time of tumor surgery.
ISSN:0095-2338
1549-960X
DOI:10.1021/ci990075q