Trypanosoma cruzi Infection Affects Actin mRNA Regulation in Heart Muscle Cells

We have previously described alterations in the cytoskeletal organization of heart muscle cells (HMC) infected with Trypanosoma cruzi in vitro. Our aim was to investigate whether these changes also affect the regulation of the actin mRNAs during HMC differentiation. Northern blot analysis revealed t...

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Bibliographic Details
Published in:The Journal of eukaryotic microbiology 2000-05, Vol.47 (3), p.271-279
Main Authors: Pereira, Mirian Claudia S, Singer, Robert H, De Meirelles, Maria De Nazareth S. L
Format: Article
Language:English
Subjects:
Actin mRNA
Actins - genetics
Actins - isolation & purification
Animals
Biological and medical sciences
cardiomyocytes
Cell Compartmentation
Cell Differentiation
Cells, Cultured
Experimental protozoal diseases and models
Gene Expression Regulation
Heart - parasitology
in situ hybridization
Infectious diseases
Medical sciences
Mice
Myocardium - cytology
Myocardium - metabolism
Parasitic diseases
Protein Isoforms - genetics
Protein Isoforms - isolation & purification
Protozoal diseases
RNA, Messenger - biosynthesis
Trypanosoma cruzi
Trypanosoma cruzi - pathogenicity
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Summary:We have previously described alterations in the cytoskeletal organization of heart muscle cells (HMC) infected with Trypanosoma cruzi in vitro. Our aim was to investigate whether these changes also affect the regulation of the actin mRNAs during HMC differentiation. Northern blot analysis revealed that α-cardiac actin mRNA levels increased during cell differentiation while β-actin mRNA levels declined. Nonmuscle cells displayed β-actin mRNA signal localized at the cell periphery, while α-cardiac actin mRNA had a perinuclear distribution in myocytes. Trypanosoma cruzi-infected cells showed 50% reduction in α-cardiac actin mRNA expression after 72 h of infection. In contrast, β-actin mRNA levels increased approximately 79% after 48 h of infection. In addition, in situ β-actin mRNA was delocalized from the periphery into the perinuclear region. These observations support the hypothesis that Trypanosoma cruzi affects actin mRNA regulation and localization through its effect on the cytoskeleton of heart muscle cells.
ISSN:1066-5234
1550-7408
DOI:10.1111/j.1550-7408.2000.tb00047.x