Investigation of binding process of chlorpromazine to bovine serum albumin by means of passive and active experiments

The association process of chlorpromazine with bovine serum albumin by both onefactoral passive and multifactoral active experiments were investigated in model systems. The dependence of absolute concentration of the free fraction of chlorpromazine on the total concentration of chlorpromazine is des...

Full description

Saved in:
Bibliographic Details
Published in:Biomedical chromatography 2001-10, Vol.15 (6), p.365-373
Main Authors: Rukhadze, Marina D., Bezarashvili, George S., Sidamonidze, Nino Sh, Tsagareli, Sonya K.
Format: Article
Language:English
Subjects:
Animals
Antipsychotic Agents - metabolism
Cattle
Chlorpromazine - metabolism
Chromatography, High Pressure Liquid
Protein Binding
Serum Albumin, Bovine - metabolism
Spectrophotometry, Ultraviolet
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The association process of chlorpromazine with bovine serum albumin by both onefactoral passive and multifactoral active experiments were investigated in model systems. The dependence of absolute concentration of the free fraction of chlorpromazine on the total concentration of chlorpromazine is described by the increasing function, but the dependence of the relative concentration (free/total) on this factor is influenced by external characteristics. The concentrations of chlorpromazine, bovine serum albumin and Tritons' mixture, as principal factors of a multifactoral experiment, influence the absolute and relative values of the free fraction of chlorpromazine non‐additively, ie the interaction of factors is significant. Copyright © 2001 John Wiley & Sons, Ltd.
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.82