Factors derived from adrenals are required for activation of cardiac gene expression in angiotensin II-induced hypertension

• Published in: Endocrinology (Philadelphia) 2001-10, Vol.142 (10), p.4256-4263
• Main Authors: Földes, G, Suo, M, Szokodi, I, Lakó-Futó, Z, deChâtel, R, Vuolteenaho, O, Huttunen, P, Ruskoaho, H, Tóth, M
• Format: Article
• Language: English
• Subjects: Adrenal Glands - physiopathology; Angiotensin II; Animals; Gene Expression Regulation; Heart - physiopathology; Hypertension - genetics; Hypertension - physiopathology; Male; Rats; Rats, Sprague-Dawley
• ISSN: 0013-7227
• DOI: 10.1210/en.142.10.4256

The mechanisms mediating the activation of cardiac gene expression during pressure overload are not fully understood. We examined whether angiotensin II-induced activation of ventricular gene expression is related to blood pressure and ventricular mass or requires other factors by infusing angiotensin II in sham-operated and adrenalectomized rats. In sham-operated rats, angiotensin II (33 microg/kg x h, sc) produced a significant increase in mean arterial pressure (measured by telemetry) within 3 h. Mean arterial pressure (up to 45 h) and the increase in left ventricular hypertrophy in adrenalectomized rats during angiotensin II infusion were similar to those in sham-operated rats. Angiotensin II produced 3.6-fold (P < 0.01) and 20.4-fold (P < 0.001) increases in ventricular atrial natriuretic peptide mRNA levels at 12 and 72 h, respectively. Angiotensin II infusion for 12 h also significantly increased the ventricular mRNA levels of B-type natriuretic peptide (5.2-fold) and adrenomedullin (1.4-fold). Adrenalectomy either abolished (atrial natriuretic peptide and adrenomedullin) or blunted (B-type natriuretic peptide) the early activation of ventricular gene expression by angiotensin II. The baseline synthesis of atrial natriuretic peptide, B-type natriuretic peptide, and adrenomedullin in the ventricle remained unchanged in adrenalectomized rats. In conclusion, our results indicate that factors derived from the adrenals are required for angiotensin II-induced early activation of cardiac gene expression.