Characterization of the differential protein expression associated with thermoresistance in human gastric carcinoma cell lines

• Published in: Electrophoresis 2001-08, Vol.22 (14), p.2990-3000
• Main Authors: Sinha, Pranav, Poland, Julia, Schnölzer, Martina, Celis, Julio E., Lage, Hermann
• Format: Article
• Language: English
• Subjects: ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism; Blotting, Western; Carcinoma - metabolism; Carcinoma - pathology; Chemoresistance; Chemotherapy; Drug Resistance, Neoplasm - genetics; Electrophoresis, Gel, Two-Dimensional; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Hot Temperature; Humans; Hyperthermia, Induced; Multidrug resistance; Neoplasm Proteins - biosynthesis; Neoplasm Proteins - genetics; Neoplasm Proteins - physiology; Proteome; Proteome analysis; Proteomics; Silver Staining; Stomach cancer; Stomach Neoplasms - metabolism; Stomach Neoplasms - pathology; Thermoresistance; Tumor Cells, Cultured - chemistry; Tumor Cells, Cultured - drug effects; Two-dimensional electrophoresis
• ISSN: 0173-0835; 1522-2683
• DOI: 10.1002/1522-2683(200108)22:14<2990::AID-ELPS2990>3.0.CO;2-F

Resistance to chemotherapeutic agents is one of the major problems faced during palliative therapy of tumor cells. Thus, chemotherapy is frequently combined with other modes of therapy such as radiation therapy and/or hyperthermia. Tumor cells respond to heat stress with development of thermotolerance and the interactions between chemo‐ and thermoresistance phenomena are not clearly understood. In this paper, we analyze the differential protein expression in vitro in human stomach cancer cells, their chemoresistant and thermoresistant counterparts using proteomics. The immediate aim was to identify sets of proteins that may lead to the development of thermoresistance. Based on these results, we aim to develop functional tests and methods for the modulation of thermoresistance and chemoresistance phenomena that may assist the therapy of inoperable cancers.