A simple, solid-phase binding assay for the nuclear import receptor karyopherin alpha. Part 1: direct binding

The nuclear import receptor karyopherin alpha recognizes nuclear localization signals (NLSs), peptides that direct the transport of proteins into the nucleus. A simple, colorimetric assay has been developed to facilitate the identification and comparison of karyopherin ligands by direct and competit...

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Bibliographic Details
Published in:Bioorganic & medicinal chemistry letters 2000-05, Vol.10 (9), p.951-954
Main Authors: Connolly, M D, Park, S B, Reedy, B M, Standaert, R F
Format: Article
Language:English
Subjects:
Alkaline Phosphatase - antagonists & inhibitors
alpha Karyopherins
Amino Acid Sequence
Antigens, Polyomavirus Transforming - chemistry
Binding, Competitive - drug effects
Biotin - chemistry
Cell Nucleus - drug effects
Cell Nucleus - metabolism
Colorimetry
Indicators and Reagents
Indoles - chemistry
Molecular Sequence Data
Nuclear Proteins - drug effects
Nuclear Proteins - metabolism
Peptides - chemical synthesis
Peptides - pharmacology
Protein Binding
Receptors, Cytoplasmic and Nuclear - drug effects
Receptors, Cytoplasmic and Nuclear - metabolism
Streptavidin
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Summary:The nuclear import receptor karyopherin alpha recognizes nuclear localization signals (NLSs), peptides that direct the transport of proteins into the nucleus. A simple, colorimetric assay has been developed to facilitate the identification and comparison of karyopherin ligands by direct and competitive binding using NLSs immobilized on the solid phase (TentaGel resin).
ISSN:0960-894X
DOI:10.1016/S0960-894X(00)00134-7