Expression of nitric oxide synthase, cyclooxygenase, and p53 in different stages of human gastric cancer

The present study evaluated the significance of nitric oxide synthase (NOS), cyclooxygenase (COX) expression and p53 status in 55 patients with gastric adenocarcinoma and relationship of these molecular markers to tumor characteristics and metastatic potential. Immunohistochemical technique was used...

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Bibliographic Details
Published in:Cancer letters 2001-10, Vol.172 (2), p.177-185
Main Authors: RAJNAKOVA, Andrea, MOOCHHALA, Shabbir, GOH, Peter M. Y, NGOI, Sing-Shang
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Biological and medical sciences
cyclooxygenase
Female
gastric carcinoma
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Immunohistochemistry
Male
Medical sciences
Middle Aged
Neoplasm Metastasis
Neoplasm Staging
Nitric Oxide Synthase - metabolism
Prostaglandin-Endoperoxide Synthases - metabolism
Stomach Neoplasms - metabolism
Stomach Neoplasms - pathology
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tumor Suppressor Protein p53 - analysis
Tumors
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Summary:The present study evaluated the significance of nitric oxide synthase (NOS), cyclooxygenase (COX) expression and p53 status in 55 patients with gastric adenocarcinoma and relationship of these molecular markers to tumor characteristics and metastatic potential. Immunohistochemical technique was used to identify the cellular location and distribution of the enzymes in the specific cells of gastric tumors. In gastric cancer tissue, the expression of inducible enzymes, iNOS and COX-2, increased significantly with increasing tumor stage (P=0.015, P=0.001, respectively), size (P=0.025, P=0.001, respectively) and the presence of metastases (P=0.002, P=0.015, respectively). The expression of constitutive enzymes, ecNOS and COX-1, followed the opposite pattern. COX-1 was significantly reduced in advanced gastric tumors (P=0.007) and tumors larger than 5 cm (P=0.007). Reduced expression of ecNOS was also observed in advanced gastric tumors; however, this did not reach statistical significance. 53% of gastric tumors showed accumulation of p53. This was significantly higher in advanced tumors (P=0.004), larger than 5 cm (P=0.015) with metastases (P
ISSN:0304-3835
1872-7980
DOI:10.1016/S0304-3835(01)00645-0