Early changes in lipid peroxidation and antioxidative defense in diabetic rat retina: effect of dl-α-lipoic acid

• Published in: European journal of pharmacology 2000-06, Vol.398 (1), p.139-146
• Main Authors: Obrosova, Irina G, Fathallah, Lamia, Greene, Douglas A
• Format: Article
• Language: English
• Subjects: Animals; Antioxidant; Antioxidants - pharmacology; Associated diseases and complications; Biological and medical sciences; Body Weight - drug effects; Diabetes Mellitus, Experimental - metabolism; Diabetes Mellitus, Experimental - prevention & control; Diabetes. Impaired glucose tolerance; Diabetic retinopathy; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Eye; Glucose - metabolism; Glutathione; Glutathione - drug effects; Glutathione - metabolism; Glutathione Disulfide - drug effects; Glutathione Disulfide - metabolism; Lipid peroxidation; Lipid Peroxidation - drug effects; Male; Medical sciences; Pharmacology. Drug treatments; Rats; Rats, Wistar; Retina - drug effects; Retina - metabolism; Superoxide dismutase; Superoxide Dismutase - drug effects; Superoxide Dismutase - metabolism; Thioctic Acid - pharmacology
• ISSN: 0014-2999; 1879-0712
• DOI: 10.1016/S0014-2999(00)00286-7

This study was designed to (1) evaluate retinal lipid peroxidation in early diabetes by the method specific for free malondialdehyde and 4-hydroxyalkenals, (2) identify impaired antioxidative defense mechanisms and (3) assess if enhanced retinal oxidative stress in diabetes is prevented by the potent antioxidant, dl-α-lipoic acid. The groups included control and streptozotocin-diabetic rats treated with or without dl-α-lipoic acid (100 mg kg −1 day −1, i.p., for 6 weeks). All parameters were measured in individual retinae. 4-Hydroxyalkenal concentration was increased in diabetic rats (2.63±0.60 vs. 1.44±0.30 nmol/mg soluble protein in controls, P