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Phenotypic and genotypic characterization of Cryptosporidium species and isolates
Recent outbreaks of cryptosporidiosis from contaminated water supplies have led to a need for the detection of Cryptosporidium oocysts from various hosts and contaminating sources. The presence of nonpathogenic species or strains of Cryptosporidium is important for diagnostic purposes as there is a...
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Published in: | Journal of industrial microbiology & biotechnology 2001-03, Vol.26 (3), p.95-106 |
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creator | CARRENO, R. A POKORNY, N. J LEE, H TREVORS, J. T DE GRANDIS, S. A |
description | Recent outbreaks of cryptosporidiosis from contaminated water supplies have led to a need for the detection of Cryptosporidium oocysts from various hosts and contaminating sources. The presence of nonpathogenic species or strains of Cryptosporidium is important for diagnostic purposes as there is a potential for false- positive detection of pathogenic parasites. The present review focuses on phenotypic differences and recent advances in genotypic analyses of the genus Cryptosporidium with an emphasis on detecting various isolates and identifying differences in Cryptosporidium parvum and other species in this genus. The information currently available demonstrates important patterns in DNA sequences of Cryptosporidium, and our understanding of macro- and microevolutionary patterns has increased in recent years. However, current knowledge of Cryptosporidium genetic diversity is far from complete, and the large amount of both phenotypic and genotypic data has led to problems in our understanding of the systematics of this genus. |
doi_str_mv | 10.1038/sj.jim.7000088 |
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A ; POKORNY, N. J ; LEE, H ; TREVORS, J. T ; DE GRANDIS, S. A</creator><creatorcontrib>CARRENO, R. A ; POKORNY, N. J ; LEE, H ; TREVORS, J. T ; DE GRANDIS, S. A</creatorcontrib><description>Recent outbreaks of cryptosporidiosis from contaminated water supplies have led to a need for the detection of Cryptosporidium oocysts from various hosts and contaminating sources. The presence of nonpathogenic species or strains of Cryptosporidium is important for diagnostic purposes as there is a potential for false- positive detection of pathogenic parasites. The present review focuses on phenotypic differences and recent advances in genotypic analyses of the genus Cryptosporidium with an emphasis on detecting various isolates and identifying differences in Cryptosporidium parvum and other species in this genus. The information currently available demonstrates important patterns in DNA sequences of Cryptosporidium, and our understanding of macro- and microevolutionary patterns has increased in recent years. However, current knowledge of Cryptosporidium genetic diversity is far from complete, and the large amount of both phenotypic and genotypic data has led to problems in our understanding of the systematics of this genus.</description><identifier>ISSN: 1367-5435</identifier><identifier>EISSN: 1476-5535</identifier><identifier>DOI: 10.1038/sj.jim.7000088</identifier><identifier>PMID: 11420647</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Animals ; Biological and medical sciences ; Biotechnology ; Cats ; Cattle ; Cryptosporidiosis ; Cryptosporidiosis - parasitology ; Cryptosporidium ; Cryptosporidium - classification ; Cryptosporidium - genetics ; Cryptosporidium - isolation & purification ; Cryptosporidium - physiology ; Food contamination & poisoning ; Fundamental and applied biological sciences. Psychology ; Genetic diversity ; Genotype ; Genotype & phenotype ; Guinea Pigs ; Humans ; Infections ; Mice ; Microbiology ; Morphology ; Oocysts ; Parasites ; Phenotype ; Protozoa ; Small intestine ; Studies ; Systematics ; Systematics. Geographical distribution. Morphology. 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A</creatorcontrib><creatorcontrib>POKORNY, N. J</creatorcontrib><creatorcontrib>LEE, H</creatorcontrib><creatorcontrib>TREVORS, J. T</creatorcontrib><creatorcontrib>DE GRANDIS, S. A</creatorcontrib><title>Phenotypic and genotypic characterization of Cryptosporidium species and isolates</title><title>Journal of industrial microbiology & biotechnology</title><addtitle>J Ind Microbiol Biotechnol</addtitle><description>Recent outbreaks of cryptosporidiosis from contaminated water supplies have led to a need for the detection of Cryptosporidium oocysts from various hosts and contaminating sources. The presence of nonpathogenic species or strains of Cryptosporidium is important for diagnostic purposes as there is a potential for false- positive detection of pathogenic parasites. The present review focuses on phenotypic differences and recent advances in genotypic analyses of the genus Cryptosporidium with an emphasis on detecting various isolates and identifying differences in Cryptosporidium parvum and other species in this genus. The information currently available demonstrates important patterns in DNA sequences of Cryptosporidium, and our understanding of macro- and microevolutionary patterns has increased in recent years. 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Psychology</subject><subject>Genetic diversity</subject><subject>Genotype</subject><subject>Genotype & phenotype</subject><subject>Guinea Pigs</subject><subject>Humans</subject><subject>Infections</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Morphology</subject><subject>Oocysts</subject><subject>Parasites</subject><subject>Phenotype</subject><subject>Protozoa</subject><subject>Small intestine</subject><subject>Studies</subject><subject>Systematics</subject><subject>Systematics. Geographical distribution. Morphology. 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subjects | Animals Biological and medical sciences Biotechnology Cats Cattle Cryptosporidiosis Cryptosporidiosis - parasitology Cryptosporidium Cryptosporidium - classification Cryptosporidium - genetics Cryptosporidium - isolation & purification Cryptosporidium - physiology Food contamination & poisoning Fundamental and applied biological sciences. Psychology Genetic diversity Genotype Genotype & phenotype Guinea Pigs Humans Infections Mice Microbiology Morphology Oocysts Parasites Phenotype Protozoa Small intestine Studies Systematics Systematics. Geographical distribution. Morphology. Cytology Taxonomy Water pollution Water supply |
title | Phenotypic and genotypic characterization of Cryptosporidium species and isolates |
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