The fine structure of the axostyle and its associations with organelles in Trichomonads

The fine structure of the axostyle in the protists Tritrichomonas foetus and Monocercomonas sp is described using transmission electron microscopy after quick-freezing techniques and immunocytochemistry. The axostyle microtubules presents a lateral projection formed by two protofilaments in addition...

Full description

Saved in:
Bibliographic Details
Published in:Tissue & cell 2000-04, Vol.32 (2), p.178-187
Main Authors: Benchimol, M., Diniz, J.A.P., Ribeiro, K.
Format: Article
Language:English
Subjects:
Animals
axostyle, pelta, microtubules, trichomonads, hydrogenosome
Cattle
Cell Nucleus - ultrastructure
Cytoskeleton - ultrastructure
Endoplasmic Reticulum, Smooth - ultrastructure
Glycogen - analysis
Immunohistochemistry
Microscopy, Electron
Microtubules - ultrastructure
Organelles - ultrastructure
Trichomonadida - cytology
Trichomonadida - ultrastructure
Tubulin - analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The fine structure of the axostyle in the protists Tritrichomonas foetus and Monocercomonas sp is described using transmission electron microscopy after quick-freezing techniques and immunocytochemistry. The axostyle microtubules presents a lateral projection formed by two protofilaments in addition to the 13 protofilaments normally found in microtubules. The axostyle is associated with other cell structures such as hydrogenosomes, endoplasmic reticulum, sigmoid filaments and glycogen particles. The microtubules of the pelta-axostylar system are connected to each other by bridges regularly spaced with an interval of 9 nm. Labeling of the axostyle was observed after cell incubation with monoclonal antibodies recognizing α-tubulin and acetylated-tubulin.
ISSN:0040-8166
1532-3072
DOI:10.1054/tice.2000.0102