Embryonic and hematopoietic stem cells express a novel SH2–containing inositol 5′-phosphatase isoform that partners with the Grb2 adapter protein

SH2–containing inositol 5′-phosphatase (SHIP) modulates the activation of immune cells after recruitment to the membrane by Shc and the cytoplasmic tails of receptors. A novel SHIP isoform of approximately 104 kd expressed in primitive stem cell populations (s-SHIP) is described. It was found that s...

Full description

Saved in:
Bibliographic Details
Published in:Blood 2001-10, Vol.98 (7), p.2028-2038
Main Authors: Tu, Zheng, Ninos, John M., Ma, Zhengyu, Wang, Jia-Wang, Lemos, Maria P., Desponts, Caroline, Ghansah, Tomar, Howson, Julie M., Kerr, William G.
Format: Article
Language:English
Subjects:
Adaptor Proteins, Signal Transducing
Animals
Base Sequence
Biological and medical sciences
Carrier Proteins - metabolism
Cell differentiation, maturation, development, hematopoiesis
Cell Line
Cell Membrane - enzymology
Cell physiology
Embryo, Mammalian - cytology
Embryo, Mammalian - enzymology
Exons
Female
Fundamental and applied biological sciences. Psychology
Hematopoietic Stem Cells - enzymology
Hematopoietic Stem Cells - metabolism
Isoenzymes - chemistry
Isoenzymes - genetics
Isoenzymes - metabolism
Male
Mice
Mice, Inbred C57BL
Molecular and cellular biology
Molecular Sequence Data
Open Reading Frames
Phosphoric Monoester Hydrolases - chemistry
Phosphoric Monoester Hydrolases - genetics
Phosphoric Monoester Hydrolases - metabolism
Protein Binding
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sequence Homology, Nucleic Acid
Signal Transduction
src Homology Domains
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:SH2–containing inositol 5′-phosphatase (SHIP) modulates the activation of immune cells after recruitment to the membrane by Shc and the cytoplasmic tails of receptors. A novel SHIP isoform of approximately 104 kd expressed in primitive stem cell populations (s-SHIP) is described. It was found that s-SHIP is expressed in totipotent embryonic stem cells to the exclusion of the 145-kd SHIP isoform expressed in differentiated hematopoietic cells. s-SHIP is also expressed in primitive hematopoietic stem cells, but not in lineage-committed hematopoietic cells. In embryonic stem cells, s-SHIP partners with the adapter protein Grb2 without tyrosine phosphorylation and is present constitutively at the cell membrane. It is postulated that s-SHIP modulates the activation threshold of primitive stem cell populations.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V98.7.2028