Downregulation of Bcl-2 and activation of caspase-8 in the UVB-induced apoptosis of a cultured human melanoma cell line

This study was performed to determine the effect of UV radiation on the activation of apoptosis regulatory proteins using cultured human melanoma cells. G361 lightly pigmented melanoma cells were irradiated with increasing doses of UVB and analyzed for an apoptotic mechanism using a cell viability t...

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Bibliographic Details
Published in:Photodermatology, photoimmunology & photomedicine photoimmunology & photomedicine, 2001-10, Vol.17 (5), p.218-222
Main Authors: PARK, K. H, CHOI, H. O, JANG, D. D, PARK, Y. I, PARK, K. C
Format: Article
Language:English
Subjects:
Apoptosis - radiation effects
Biological and medical sciences
Blotting, Western
Caspase 8
Caspase 9
Caspases - metabolism
Chi-Square Distribution
Dermatology
Down-Regulation
Enzyme Activation - radiation effects
Flow Cytometry
Humans
Medical sciences
Melanoma - metabolism
Melanoma - pathology
Microscopy, Electron
Proto-Oncogene Proteins c-bcl-2 - metabolism
Tumor Cells, Cultured
Tumors of the skin and soft tissue. Premalignant lesions
Ultraviolet Rays - adverse effects
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Summary:This study was performed to determine the effect of UV radiation on the activation of apoptosis regulatory proteins using cultured human melanoma cells. G361 lightly pigmented melanoma cells were irradiated with increasing doses of UVB and analyzed for an apoptotic mechanism using a cell viability test, TEM, FACS, and western blotting analysis. TEM and FACS showed apoptotic features of cell death after UVB irradiation. Western blotting disclosed downregulation of Bcl-2 and the activation of caspase-9. Caspase-8, a downstream molecule of Fas/FasL interaction, was also activated. The activation of downstream molecules of both caspase-8 and caspase-9 was also demonstrated. Our data showed that the regulation of the Bcl-2 family and caspase-8 may work together to activate a caspase-3 mediated apoptotic pathway following UVB irradiation of cultured human melanoma cells.
ISSN:0905-4383
1600-0781
DOI:10.1034/j.1600-0781.2001.170503.x