Development of an ELISA system for Detection of Anti-Anaplasma marginale Antibodies in cattle in Brazil

Summary An ELISA test was developed for detecting antibodies against Anaplasma marginale in bovine sera. Four antigenic preparations were produced from infected red blood cells. Some aliquots of this preparation were stored at ‐70°C with 30% DMSO in phosphate‐buffered saline (PBS) and others were ly...

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Bibliographic Details
Published in:Journal of veterinary medicine. Series B 2000-05, Vol.47 (4), p.241-248
Main Authors: Braz jr, C.J., Ribeiro, M.F.B., Lima, J.D., Passos, L.M.F.
Format: Article
Language:English
Subjects:
Anaplasma - immunology
Anaplasma - isolation & purification
Anaplasma marginale
Anaplasmosis - diagnosis
Anaplasmosis - immunology
Anaplasmosis - microbiology
Animals
Animals, Newborn
Antibodies, Bacterial - blood
Cattle
Cattle Diseases - diagnosis
Cattle Diseases - microbiology
Enzyme-Linked Immunosorbent Assay - veterinary
Sensitivity and Specificity
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Description
Summary:Summary An ELISA test was developed for detecting antibodies against Anaplasma marginale in bovine sera. Four antigenic preparations were produced from infected red blood cells. Some aliquots of this preparation were stored at ‐70°C with 30% DMSO in phosphate‐buffered saline (PBS) and others were lysed with 0.9% NH4Cl and stored at ‐ 20°C. Typical anaplasmal structures were seen by electron microscopy in the antigenic preparations containing the erythrocytes that had been stored with DMSO. The performance of the ELISA test was evaluated by testing 298 positive serum samples collected from immunized cattle, 39 negative serum samples collected from cattle imported from areas free of A. marginale and 50 samples collected from cattle naturally infected in the field. The test gave a specificity of 94.87% and a sensitivity of 100%.
ISSN:0931-1793
1439-0450
DOI:10.1046/j.1439-0450.2000.00342.x